Structure of PDB 8hhl Chain A Binding Site BS02

Receptor Information

>8hhl Chain A (length=592) Species: 56689 (Mycolicibacterium mucogenicum)

MTTMTVHTMGVHYKWQIPEVLRQQLWLAHNLREDLVSLQLAYDDDLKAIW
SSYPDVAQAEDTMAAAEADAVALSERVKQARIEARSKKISTELTQQLRDA
KKRLKDARQARRDAIAVVKDDAAERRKARSDQLAADQKALYGQYCRDGDL
YWASFNTVLDHHKTAVKRIAAQRASGKPATLRHHRFDGSGTIAVQLQRQA
GAPPRTPMVLADEAGKYRNVLHIPGWTDPDVWEQMTRSQCRQSGRVTVRM
RCGSTDGQPQWIDLPVQVHRWLPADADITGAELVVTRVAGIYRAKLCVTA
RIGDTEPVTSGPTVALHLGWRSTEEGTAVATWRSDAPLDIPFGLRTVMRV
DAAGTSGIIVVPATIERRLTRTENIASSRSLALDALRDKVVGWLSDNDAP
TYRDAPLEAATVKQWKSPQRFASLAHAWKDNGTEISDILWAWFSLDRKQW
AQQENGRRKALGHRDDLYRQIAAVISDQAGHVLVDDTSVAELSARAMERT
ELPTEVQQKIDRRRDHAAPGGLRASVVAAMTRDGVPVTIVAAADFTRTHS
RCGHVNPADDRYLSNPVRCDGCGAMYDQDRSFVTLMLRAATA

Ligand information

>8hhl Chain C (length=31)

tgccacgcgcacctcatctcccaaatagaca

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8hhl Mechanistic and evolutionary insights into a type V-M CRISPR-Cas effector enzyme.
• Resolution: 2.87 Å
• Binding residue (original residue number in PDB): M4 K78 R81 I82 R85 S86 K87 R168 Q195 Q197 Q199 A200 T279 K416 S417 Q419 W450 E454 R457 L461 R464 S488 V489 A490 P503 T504 A518 G520
• Binding residue (residue number reindexed from 1): M4 K78 R81 I82 R85 S86 K87 R168 Q195 Q197 Q199 A200 T279 K416 S417 Q419 W450 E454 R457 L461 R464 S488 V489 A490 P503 T504 A518 G520

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0046872 metal ion binding

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:8hhl, PDBe:8hhl, PDBj:8hhl
• PDBsum: 8hhl
• PubMed: 37460897
• UniProt: P0DXB1|CS12M_MYCMU CRISPR-associated DNA-binding protein Cas12m (Gene Name=cas12m)