Structure of PDB 8h1j Chain A Binding Site BS02

Receptor Information

>8h1j Chain A (length=362) Species: 243230 (Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539)

MIRNKAFVVRLYPNAAQTELINRTLGSARFVYNHFLARRIAAYKESGKGL
TYGQTSSELTLLKQAEETSWLSEVDKFALQNSLKNLETAYKNFFRTVKQS
GKKVGFPRFRKKRTGESYRTQFTNNNIQIGEGRLKLPKLGWVKTKGQQDI
QGKILNVTVRRIHEGHYEASVLCEVEIPYLPAAPKFAAGVDVGIKDFAIV
TDGVRFKHEQNPKYYRSTLKRLRKAQQTLSRRKKGSARYGKAKTKLARIH
KRIVNKRQDFLHKLTTSLVREYEIIGTEHLDAGWGEFIRQLEYKAAWYGR
LVSKVSPYFPSSQLCHDCGFKNPEVKNLAVRTWTCPNCGETHDRDENAAL
NIRREALVAAGI

Ligand information

>8h1j Chain B (length=102)

gguggcugcgggaaucucagacaccuuaaacgcucauggaggcuauaugg
ucugcgaagugagaaucacgcgacgucgugugagguucaagagucccuug
gc

<<<<.<<<...((((..>>>.>>>>.......<<<<<...<<<<<..>>>
>>......>>>>>..<<<<<<<<..>>>>>>>>.))))............
..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8h1j Cryo-EM structure of the transposon-associated TnpB enzyme.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): N4 A6 V8 A89 N92 G105 R110 S117 K143 G146 Q148 R160 S170 Y214 K220 R221 R223 Q227 R231 R232 K234 S236 A237 R238 K256 D259 H262 K263 T266 R270 Y309 W313
• Binding residue (residue number reindexed from 1): N4 A6 V8 A89 N92 G105 R110 S117 K143 G146 Q148 R160 S170 Y214 K220 R221 R223 Q227 R231 R232 K234 S236 A237 R238 K256 D259 H262 K263 T266 R270 Y293 W297

Enzymatic activity

• Enzyme Commision number: 3.1.21.-

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006310 DNA recombination
• GO:0032196 transposition

External links

• PDB: RCSB:8h1j, PDBe:8h1j, PDBj:8h1j
• PDBsum: 8h1j
• PubMed: 37020030
• UniProt: Q7DF80|DRA2B_DEIRA RNA-guided DNA endonuclease TnpB (Gene Name=tnpB)