Structure of PDB 8fs2 Chain A Binding Site BS02

Receptor Information

>8fs2 Chain A (length=543) Species: 1496 (Clostridioides difficile)

YYTPKIIVDYIVKKTLKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDL
FEENIYELKKKYDENYWTVDNIHRHILNYCIYGADIDEKAISILKDSLTN
KKVESDIKINLFCCDSLKKKWRYKFDYIVGNPPYIGHKKLEKKYKKFLLE
KYSEVYKDKADLYFCFYKKIIDILKQGGIGSVITPRYFLESLSGKDLREY
IKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYIDVFKIKNED
ICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINK
YIVDKSEYRLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKW
YELQWGREKLFFERKKIMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEY
LDKFSYEYLVGILNSSVYDKYFKITAKKMSKNIYDYYPNKVMKIRIFRDN
NYEEIENLSKQIISILLNKSIDKGKVEKLQIKMDNLIMDSLGI

Ligand information

>8fs2 Chain E (length=14)

atgggactttttga

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fs2 Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile- Specific DNA Adenine Methyltransferase.
• Resolution: 2.59 Å
• Binding residue (original residue number in PDB): H171 K172 K176 K179 D192 K193 I349 G351 C352 D353 K378 K380 R425 Q438 W439 Y455
• Binding residue (residue number reindexed from 1): H137 K138 K142 K145 D158 K159 I315 G317 C318 D319 K344 K346 R391 Q404 W405 Y421

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0032259 methylation

External links

• PDB: RCSB:8fs2, PDBe:8fs2, PDBj:8fs2
• PDBsum: 8fs2
• PubMed: 37082867
• UniProt: Q183J3