Structure of PDB 8dga Chain A Binding Site BS02

Receptor Information
>8dga Chain A (length=1629) Species: 7227 (Drosophila melanogaster) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
NLHTTVFTPRDFQVELLATAYERNTIICLGHRSSKEFIALKLLQELSRRA
RRHGRVSVYLSCEVGTSTEPCSIYTMLTHLTDLRVWQEQPDMQIPFDHCW
TDYHVSILRPEGFLYLLETRELLLSRVELIVLEDCHDSAVYQRIRPLFEN
HIMPAPPADRPRILGLAGPLHSAGCELQQLSAMLATLEQSVLCQIESASD
IVTVLRYCSRPHEYIVQCAPFEMDELSLVLADVLNTHKSFLLDHRYDDPL
NVINSLLVVLHEMGPWCTQRAAHHFYQCNEKLKVKTPHERHYLLYCLVST
ALIQLYSLCEHAFHRHLSRQTIERYSSPKVRRLLQTLRCFKTLCALIYCN
QNHTARVLFELLAEISRRDPDLKFLRCTEPKEAELEHRRQEEVLKRFRML
IGTSVLEEGIDVPKCNLVVRWDPPTTYRSYVQCKGRARAAPAYHVILVAS
TTKDLVHQMAQYREIEQMLLSKCANTEPPEQEQCEAERFSACLAAYRPKP
HLLTGASVDLGSAIALVNKYCARLPSDTFTKLTALWRCTRNERAGVTLFQ
YTLRLPINSPLKHDIVGLPMPTQTLARRLAALQACVELHRIGELDKEGFR
ALEPDWECFELEPEDEQIVQLSDEPRPGTTKRRQYYYKRIASEFCDCRPV
AGAPCYLYFIQLTLQCPIPEEQNTRGRKIYPPEDAQQGFGILTTKRIPKL
SAFSIFTRSGEVKVSLELAKERVILTSEQIVCINGFLNYTFTNVLRLQKF
LMLFDPDSTENCVFIVPTVKAPAGGKHIDWQFLELIQANGNTMPRAVPDE
ERQAQPFDPQRFQDAVVMPWYRNQDQPQYFYVAEICPHLSPLSCFPGDNY
RTFKHYYLVKYGLTIQNTSQPLLDVDHTSARLNFLTPRYVNRKGVALPTS
SEETKRAKRENLEQKQILVPELCTVHPFPASLWRTAVCLPCILYRINGLL
LADDIRKQVSADLGLGRQQIEDEDFEWPMLDFGWSLANERQYQQTKNLLI
GFNFKHEDQKEPATIRYEESIAKLKTEIESGGMLVPHDQQLVLKRKVSMM
ELLKQLLPYVNEDVLAKKLGDRRELLLSDLVELNADWVARHEQETYNVMG
CGDSFDNYNDHHRLNLDEKQLKLPAGFSFDRQPDLVGHPGPSPSIILQAL
TMSNANDGINLERLETIGDSFLKYAITTYLYITYENVHEGKLSHLRSKQV
ANLNLYRLGRRKRLGEYMIATKFEPHDNWLPPCYYVPKELEKALIEAKIP
THHWKLADLLDIKNLSSVQICEMVREKPYNLVSQHSIPDKSIADCVEALI
GAYLIECGPRGALLFMAWLGVRVLPITRQLDGGNQEQRIPGSTKPNAENV
VTVYGAWPTPRSPLLHFAPNATEELDQLLSGFEEFEESLGYKFRDRSYLL
QAMTHASYTPNRLTDCYQRLEFLGDAVLDYLITRHLYEDPRQHSPGALTD
LRSALVNNTIFASLAVRHGFHKFFRHLSPGLNDVIDRFVRIQQENGHCIS
EEYYLLSEEECDDAEDVEVPKALGDVFESIAGAIFLDSNMSLDVVWHVYS
NMMSPEIEQFSNSVPKSPIRELLELEPETAKFGKPEKLADGRRVRVTVDV
FCKGTFRGIGRNYRIAKCTAAKCALRQLK
Ligand information
>8dga Chain F (length=22) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cuauacaaccuacuaccucucu
......................
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8dga Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB.
Resolution3.73 Å
Binding residue
(original residue number in PDB)
R1141 Y1175 Y1176 K1179 R1207 L1216 P1217 T1218 S1219 S1220 T1223 E1232 Q1235 I1236 T2060 D2061 R2181
Binding residue
(residue number reindexed from 1)
R822 Y856 Y857 K860 R888 L897 P898 T899 S900 S901 T904 E913 Q916 I917 T1449 D1450 R1570
Enzymatic activity
Enzyme Commision number 3.1.26.3: ribonuclease III.
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003727 single-stranded RNA binding
GO:0004386 helicase activity
GO:0004519 endonuclease activity
GO:0004521 RNA endonuclease activity
GO:0004525 ribonuclease III activity
GO:0004530 deoxyribonuclease I activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016891 RNA endonuclease activity, producing 5'-phosphomonoesters
GO:0046872 metal ion binding
GO:0070883 pre-miRNA binding
Biological Process
GO:0006309 apoptotic DNA fragmentation
GO:0006396 RNA processing
GO:0007279 pole cell formation
GO:0007294 germarium-derived oocyte fate determination
GO:0007367 segment polarity determination
GO:0030422 siRNA processing
GO:0030727 germarium-derived female germ-line cyst formation
GO:0031047 regulatory ncRNA-mediated gene silencing
GO:0031054 pre-miRNA processing
GO:0033227 dsRNA transport
GO:0035194 regulatory ncRNA-mediated post-transcriptional gene silencing
GO:0035196 miRNA processing
GO:0042078 germ-line stem cell division
GO:0042594 response to starvation
GO:0045448 mitotic cell cycle, embryonic
GO:0048813 dendrite morphogenesis
GO:0070922 RISC complex assembly
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016442 RISC complex
GO:0070578 RISC-loading complex

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Molecular Function

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Biological Process

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Cellular Component
External links
PDB RCSB:8dga, PDBe:8dga, PDBj:8dga
PDBsum8dga
PubMed36182693
UniProtQ9VCU9|DCR1_DROME Endoribonuclease Dcr-1 (Gene Name=Dcr-1)

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