Structure of PDB 8d4a Chain A Binding Site BS02

Receptor Information
>8d4a Chain A (length=1207) Species: 1539066 (Sulfuricurvum sp. PC08-66) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MLHAFTNQYQLSKTLRFGATLKEDEKKCKSHEELKGFVDISYENMKSSAT
ENELVKKCERCYSEIVKFHNAWEKIYYRTDQIAVYKDFYRQLSRKARFDA
GKQNSQLITLASLCGMYQGAKLSRYITNYWKDNITRQKSFLKDFSQQLHQ
YTRALEKSDKAHTKPNLINFNKTFMVLANLVNEIVIPLSNGAISFPNISK
LEDGEESHLIEFALNDYSQLSELIGELKDAIATNGGYTPFAKVTLNHYTA
EQKPHVFKNDIDAKIRELKLIGLVETLKGKSSEQIEEYFSNLDKFSTYND
RNQSVIVRTQCFKYKPIPFLVKHQLAKYISEPNGWDEDAVAKVLDAVGAI
RSPAHDYANNQEGFDLNHYPIKVAFDYAWEQLANSLYTTVTFPQEMCEKY
LNSIYGCEVSKEPVFKFYADLLYIRKNLAVLEHKNNLPSNQEEFICKINN
TFENIVLPYKISQFETYKKDILAWINDGHDHKKYTDAKQQLGFIRGGLKG
RINPYTKLTNEFKQISSTYGKTFAELRDKFKEKNEITKITHFGIIIEDKN
RDRYLLASELKHEQINHVSTILNKLDKSSEFITYQVKSLTSKTLIKLIKN
HTTKKGAISPYADFHTSKTGFNKNEIEKNWDNYKREQVLVEYVKDCLTDS
TMAKNQNWAEFGWNFEKCNSYEDIEHEIDQKSYLLQSDTISKQSIASLVE
GGCLLLPIINQDITSKERKDKNQFSKDWNHIFEGSKEFRLHPEFAVSYRT
PIEGYPVQKRYGRLQFVCAFNAHIVPQNGEFINLKKQIENFNDEDVQKRN
VTEFNKKVNHALSDKEYVVIGIDRGLKQLATLCVLDKRGKILGDFEIYKK
EFVRAEKRSESHWEHTQAETRHILDLSNLRVETTIEGKKVLVDQSLTLVK
KNRDTPDEEATEENKQKIKLKQLSYIRKLQHKMQTNEQDVLDLINNEPSD
EEFKKRIEGLISSFGEGQKYADLPINTMREMISDLQGVIARGNNQTEKNK
IIELDAADNLKQGIVANMIGIVNYIFAKYSYKAYISLEDLSRAYGGAKSG
YDGRYLPSTSQDEDVDFKEQQNQMLAGLGTYQFFEMQLLKKLQKIQSDNT
VLRFVPAFRSADNYRNILRLEETKYKSKPFGVVHFIDPKFTSKKCPVCSK
TNVYRDKDDILVCKECGFRSDSQLKERENNIHYIHNGDDNGAYHIALKSV
ENLIQMK
Ligand information
>8d4a Chain C (length=28) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
agccuucuucagguguugcuuuagaaag
............................
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8d4a RNA targeting unleashes indiscriminate nuclease activity of CRISPR-Cas12a2.
Resolution2.74 Å
Binding residue
(original residue number in PDB)		R103 Y123 H168 K170 L173 Y254 K259 H261 V262 D382 E386 R431 A435 H439 K546 E550 K554 S613 T615 K617 T618 K621 N625 T627 Y636 K648 Q681 H766 P767 E768 I813 R952 E1028 L1029 A1031 A1032 K1036 E1094 N1097 Q1107 R1134 S1135 A1136 D1137
Binding residue
(residue number reindexed from 1)		R97 Y117 H162 K164 L167 Y248 K253 H255 V256 D376 E380 R425 A429 H433 K521 E525 K529 S588 T590 K592 T593 K596 N600 T602 Y611 K623 Q656 H741 P742 E743 I788 R927 E1003 L1004 A1006 A1007 K1011 E1069 N1072 Q1082 R1109 S1110 A1111 D1112
Enzymatic activity
Enzyme Commision number ?
Gene Ontology

View graph for
Molecular Function
External links
PDB RCSB:8d4a, PDBe:8d4a, PDBj:8d4a
PDBsum8d4a
PubMed36599980
UniProtA0A0C2W1L1

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