Structure of PDB 7sp3 Chain A Binding Site BS02

Receptor Information

>7sp3 Chain A (length=153) Species: 562 (Escherichia coli)

MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGESAEQAM
YRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVCIGQKQKWFLLQLVSG
DAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMS
LAA

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 7sp3 Chain A Residue 202

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7sp3 A distinct RNA recognition mechanism governs Np 4 decapping by RppH.
• Resolution: 1.6 Å
• Binding residue (original residue number in PDB): Q37 E57 E120
• Binding residue (residue number reindexed from 1): Q37 E57 E113
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 3.6.1.-

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0005515 protein binding
• GO:0016462 pyrophosphatase activity
• GO:0016787 hydrolase activity
• GO:0016818 hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
• GO:0034353 mRNA 5'-diphosphatase activity
• GO:0110153 RNA NAD-cap (NMN-forming) hydrolase activity

Biological Process

• GO:0006402 mRNA catabolic process
• GO:0008033 tRNA processing
• GO:0050779 RNA destabilization
• GO:0110154 RNA decapping
• GO:0110155 NAD-cap decapping

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7sp3, PDBe:7sp3, PDBj:7sp3
• PDBsum: 7sp3
• PubMed: 35131855
• UniProt: P0A776|RPPH_ECOLI RNA pyrophosphohydrolase (Gene Name=rppH)