Structure of PDB 7sh2 Chain A Binding Site BS02

Receptor Information

>7sh2 Chain A (length=405) Species: 4932 (Saccharomyces cerevisiae)

GEQWYEKFKPNCLEQVAIHKRKLKDVQEALDAMFLPNAKHRILLLSGPSG
CSKSTVIKELSKILVPKYHKVTEFRGQMESFSEFLKGARYLVMSNLSLIL
IEDLPNVFHIDTRRRFQQLILQWLYSSEPLLPPLVICITECEIPENKFGI
DYTFSAETIMNKEILMHPRLKRIKFNPINSTLLKKHLKFICVQNMKMLKE
KNKWNKRQEVIDYIAQETGDIRSAITTLQFWATSSGSLPISTRESTISYF
HAIGKVIHGSHSTNNDNEMINNLFENSNNLLSKEDFKLGILENYNTFNKG
EFSISDASSIVDCLSECDNMNGLPESNEYGLREVRKTFRNISKQGHNHGT
VYFPREWKVRKLQNSFKVQAEDWLNVSLYKYNAVHSFRNITLEFGYYAPL
IRKCQ

Ligand information

>7sh2 Chain T (length=13)

tagtgtctgcttt

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7sh2 DNA is loaded through the 9-1-1 DNA checkpoint clamp in the opposite direction of the PCNA clamp.
• Resolution: 3.23 Å
• Binding residue (original residue number in PDB): H81 R83 K84 E247 K252 N266 N269 T271 Y339 F340 V441 Y442 F443 W447
• Binding residue (residue number reindexed from 1): H19 R21 K22 E157 K162 N176 N179 T181 Y249 F250 V351 Y352 F353 W357

Gene Ontology

Molecular Function

• GO:0003682 chromatin binding
• GO:0003689 DNA clamp loader activity
• GO:0005515 protein binding
• GO:0005524 ATP binding

Biological Process

• GO:0000077 DNA damage checkpoint signaling
• GO:0006281 DNA repair
• GO:0006289 nucleotide-excision repair
• GO:0006974 DNA damage response
• GO:0007131 reciprocal meiotic recombination
• GO:0033314 mitotic DNA replication checkpoint signaling

Cellular Component

• GO:0005634 nucleus
• GO:0031389 Rad17 RFC-like complex

External links

• PDB: RCSB:7sh2, PDBe:7sh2, PDBj:7sh2
• PDBsum: 7sh2
• PubMed: 35314830
• UniProt: P32641|RAD24_YEAST Checkpoint protein RAD24 (Gene Name=RAD24)