Structure of PDB 7mid Chain A Binding Site BS02

Receptor Information

>7mid Chain A (length=554) Species: 243231 (Geobacter sulfurreducens PCA)

DGSIPLIPVRMLNEHVYCPRLAYLMWVQGEFSHNEFTVDGVIRHRRVDAG
GGVLPSETQEDSRIHARSVSLSSERLGITAKIDLVEGEGAYVSPVDYKRG
KRPHVAGGAYEPERVQLCAQGLLLREHGFASDGGALYFVASRERVPVAFD
DELIGRTLAAIDEMGRTALSGTMPPPLEDSPKCPRCSLVGICLPDEVRFL
SHLSVEPRPIIPADGRGLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARL
GETSQVALFGNATLTTAALHECLRREIPVTWLSYGGWFMGHTVSTGHRNV
ETRTYQYQRSFDPETCLNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPP
GLLMSLQDDMRHAMRAPSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMG
FDFTTRNRRPPKDPVNALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPR
FGRPALALDMMEPFRPLIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSA
RKRFIAGFERRMEQEVTHPIFKYTISYRRLLEVQARLLTRYLSGEIPAYP
NFVT

Ligand information

>7mid Chain F (length=33)

cgtagctgaggcctcacgatggactttttgaat

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7mid Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
• Resolution: 3.56 Å
• Binding residue (original residue number in PDB): R14 E18 Y21 M29 F35 F40 T41 G44 H48 K85 K102 R103 Y114 R189 C190 S191
• Binding residue (residue number reindexed from 1): R10 E14 Y17 M25 F31 F36 T37 G40 H44 K81 K98 R99 Y110 R185 C186 S187

Enzymatic activity

• Enzyme Commision number: 3.1.-.-
  3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004527 exonuclease activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:7mid, PDBe:7mid, PDBj:7mid
• PDBsum: 7mid
• PubMed: 34588691
• UniProt: Q74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)