Structure of PDB 7mi5 Chain A Binding Site BS02

Receptor Information

>7mi5 Chain A (length=553) Species: 243231 (Geobacter sulfurreducens PCA)

GSIPLIPVRMLNEHVYCPRLAYLMWVQGEFSHNEFTVDGVIRHRRVDAGG
GVLPSETQEDSRIHARSVSLSSERLGITAKIDLVEGEGAYVSPVDYKRGK
RPHVAGGAYEPERVQLCAQGLLLREHGFASDGGALYFVASRERVPVAFDD
ELIGRTLAAIDEMGRTALSGTMPPPLEDSPKCPRCSLVGICLPDEVRFLS
HLSVEPRPIIPADGRGLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARLG
ETSQVALFGNATLTTAALHECLRREIPVTWLSYGGWFMGHTVSTGHRNVE
TRTYQYQRSFDPETCLNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPPG
LLMSLQDDMRHAMRAPSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMGF
DFTTRNRRPPKDPVNALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPRF
GRPALALDMMEPFRPLIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSAR
KRFIAGFERRMEQEVTHPIFKYTISYRRLLEVQARLLTRYLSGEIPAYPN
FVT

Ligand information

>7mi5 Chain H (length=34)

tcgtagctgaggcctcacgatggactttttgaat

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7mi5 Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
• Resolution: 3.57 Å
• Binding residue (original residue number in PDB): V13 R14 E18 Y21 M29 F35 F40 T41 G44 H48 K85 D87 K102 R103 Y114 P185 R189 C190 S191 R278
• Binding residue (residue number reindexed from 1): V8 R9 E13 Y16 M24 F30 F35 T36 G39 H43 K80 D82 K97 R98 Y109 P180 R184 C185 S186 R273

Enzymatic activity

• Enzyme Commision number: 3.1.-.-
  3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004527 exonuclease activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:7mi5, PDBe:7mi5, PDBj:7mi5
• PDBsum: 7mi5
• PubMed: 34588691
• UniProt: Q74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)