Structure of PDB 6wc0 Chain A Binding Site BS02

Receptor Information

>6wc0 Chain A (length=938) Species: 351607 (Acidothermus cellulolyticus 11B)

GTVPVTWRLGVDVGERSIGLAAVSYEEDKPKEILAAVSWIHDGGVGDERS
GASRLALRGMARRARRLRRFRRARLRDLDMLLSELGWTPLPDKNVSPVDA
WLARKRLAEEYVVDETERRRLLGYAVSHMARHRGWRNPWTTIKDLKNLPQ
PSDSWERTRESLEARYSVSLEPGTVGQWAGYLLQRAPGIRLNPTQQSLSN
ATAFETRLRQEDVLWELRCIADVQGLPEDVVSNVIDAVFCQKRPSVPAER
IGRDPLDPSQLRASRACLEFQEYRIVAAVANLRIRDGSGSRPLSLEERNA
VIEALLAQTERSLTWSDIALEILKLPNESDLTSVPEEDGPSSLAYSQFAP
FDETSARIAEFIAKNRRKIPTFAQWWQEQDRTSRSDLVAALADNSIAGHL
PDAELEALEGLALPSGRVAYSRLTLSGLTRVMRDDGVDVHNARKTCFGVD
DNWRPPLPALHEATGHPVVDRNLAILRKFLSSATMRWGPPQSIVVELGSA
GGYAAVALRDRLLSYGEKNGVAQVAVFRGGVTAEARRWLDISIERLFSRV
AIFAQSTSTKRLDRRHHAVDAVVLTTLTPGVAKTLADARSRRVSASTEEP
QSPAYRQWKESCSGLGDLLISTAARDSIAVAAPLRLRPTGALHEETLRAF
SEHTVGAAWKGAELRRIVEPEVYAAFLALTDPGGRFLKVSPSEDVLPADE
NRHIVLSDRVLGPRDRVKLFPDDRGSIRVRGGAAYIASFHHARVFRWGSS
HSPSFALLRVSLADLAVAGLLRDGVDVFTAELPPWTPAWRYASIALVKAV
ESGDAKQVGWLVPGDELDFGPEGVTTAAGDLSMFLKYFPERHWVVTGFED
DKRINLKPAFLSAEQAEVLRTERSDRPDTLTEAGEILAQFFPRCWRATVA
KVLCHPGLTVIRRTALGQPRWRRGHLPYSWRPWSADPW

Ligand information

>6wc0 Chain C (length=30)

cgccagatatataccaggatcttgccatcc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6wc0 The molecular basis for recognition of 5'-NNNCC-3' PAM and its methylation state by Acidothermus cellulolyticus Cas9.
• Resolution: 3.61 Å
• Binding residue (original residue number in PDB): R55 W141 W145 R219 R262 G264 R274 L355 A356 Y357 S358 Q359 I408 R437 E839 E840 T841 Q1084 R1088 R1091
• Binding residue (residue number reindexed from 1): R49 W135 W139 R207 R250 G252 R262 L343 A344 Y345 S346 Q347 I396 R417 E644 E645 T646 Q889 R893 R896

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding

External links

• PDB: RCSB:6wc0, PDBe:6wc0, PDBj:6wc0
• PDBsum: 6wc0
• PubMed: 33311465
• UniProt: A0LWB3