Structure of PDB 6wbr Chain A Binding Site BS02

Receptor Information

>6wbr Chain A (length=942) Species: 351607 (Acidothermus cellulolyticus 11B)

GTVPVTWRLGVDVGERSIGLAAVSYEEDKPKEILAAVSWIHDGGVGDERS
GASRLALRGMARRARRLRRFRRARLRDLDMLLSELGWTPLPDKNVSPVDA
WLARKRLAEEYVVDETERRRLLGYAVSHMARHRGWRNPWTTIKDLKNLPQ
PSDSWERTRESLEARYSVSLEPGTVGQWAGYLLQRAPGIRLNPTQQSLSN
ATAFETRLRQEDVLWELRCIADVQGLPEDVVSNVIDAVFCQKRPSVPAER
IGRDPLDPSQLRASRACLEFQEYRIVAAVANLRIRDGSGSRPLSLEERNA
VIEALLAQTERSLTWSDIALEILKLPNESDLTSVPEEDGPSSLAYSQFAP
FDETSARIAEFIAKNRRKIPTFAQWWQEQDRTSRSDLVAALADNSIAGLL
VHLPDAELEALEGLALPSGRVAYSRLTLSGLTRVMRDDGVDVHNARKTCF
GVDDNWRPPLPALHEATGHPVVDRNLAILRKFLSSATMRWGPPQSIVVEL
GSAGGYAAVALRDRLLSYGEKNGVAQVAVFRGGVTAEARRWLDISIERLF
SRVAIFAQSTSTKRLDRRHHAVDAVVLTTLTPGVAKTLADARSRRVSAST
EEPQSPAYRQWKESCSGLGDLLISTAARDSIAVAAPLRLRPTGALHEETL
RAFSEHTVGAAWKGAELRRIVEPEVYAAFLALTDPGGRFLKVSPSEDVLP
ADENRHIVLSDRVLGPRDRVKLFPDDRGSIRVRGGAAYIASFHHARVFRW
GSSHSPSFALLRVSLADLAVAGLLRDGVDVFTAELPPWTPAWRYASIALV
KAVESGDAKQVGWLVPGDELDFGPEGVTTAAGDLSMFLKYFPERHWVVTG
FEDDKRINLKPAFLSAEQAEVLRTERSDRPDTLTEAGEILAQFFPRCWRA
TVAKVLCHPGLTVIRRTALGQPRWRRGHLPYSWRPWSADPWS

Ligand information

>6wbr Chain C (length=30)

cgccaggtatataccaggatcttgccatcc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6wbr The molecular basis for recognition of 5'-NNNCC-3' PAM and its methylation state by Acidothermus cellulolyticus Cas9.
• Resolution: 2.91 Å
• Binding residue (original residue number in PDB): R55 W141 W145 R219 G264 R274 A356 Y357 S358 I408 R437 Y686 E839 E840 T841 D1025 K1047 R1088 R1091 T1093 K1096
• Binding residue (residue number reindexed from 1): R49 W135 W139 R207 G252 R262 A344 Y345 S346 I396 R420 Y506 E647 E648 T649 D833 K855 R896 R899 T901 K904

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding

External links

• PDB: RCSB:6wbr, PDBe:6wbr, PDBj:6wbr
• PDBsum: 6wbr
• PubMed: 33311465
• UniProt: A0LWB3