Structure of PDB 6uk0 Chain A Binding Site BS02

Receptor Information

>6uk0 Chain A (length=532) Species: 11706 (HIV-1 M:B_HXB2R)

SPIETVPVKLKPGMDGPKVKQEKIKALVEICTEMEKEGKISKIGPENPYN
TPVFAIWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDA
YFSVPLDEDFRKYTAFTIPSIIRYQYNVLPQGWKGSPAIFQSSMTKILEP
FRKQNPDIVIYQYVDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKK
HQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDICKLVGKLNWASQI
YPGIKVRQLSKLLRGTEVIPLTEEAELELAENREILKEPVHGVYYDPSKD
LIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQK
ITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTPPLVK
LWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTN
QKTELQAIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQ
LIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGI

Ligand information

>6uk0 Chain T (length=20)

gggggcgcccgaacagggac

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6uk0 Elucidating molecular interactions ofL-nucleotides with HIV-1 reverse transcriptase and mechanism of M184V-caused drug resistance.
• Resolution: 2.75695 Å
• Binding residue (original residue number in PDB): L74 R78 E89 Q91 I94 G152 P157 S280 L283 R284 G285 K374 Q500
• Binding residue (residue number reindexed from 1): L60 R64 E75 Q77 I80 G132 P137 S260 L263 R264 G265 K350 Q476

Enzymatic activity

• Enzyme Commision number: 2.7.7.-
  2.7.7.49: RNA-directed DNA polymerase.
  2.7.7.7: DNA-directed DNA polymerase.
  3.1.-.-
  3.1.13.2: exoribonuclease H.
  3.1.26.13: retroviral ribonuclease H.
  3.4.23.16: HIV-1 retropepsin.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003964 RNA-directed DNA polymerase activity
• GO:0004523 RNA-DNA hybrid ribonuclease activity

Biological Process

• GO:0006278 RNA-templated DNA biosynthetic process

External links

• PDB: RCSB:6uk0, PDBe:6uk0, PDBj:6uk0
• PDBsum: 6uk0
• PubMed: 31872074
• UniProt: P04585|POL_HV1H2 Gag-Pol polyprotein (Gene Name=gag-pol)