Structure of PDB 6juq Chain A Binding Site BS02

Receptor Information

>6juq Chain A (length=341) Species: 562 (Escherichia coli)

SRKIIHVDMDCFAAAVEMRDNPALRDIPIAIGGSRERRGVISCANYPARK
FGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPL
SLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAK
IASDMNKPNGQFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTC
GDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRKSVGVERTMA
EDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQ
EHVWPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLLDP

Ligand information

>6juq Chain B (length=17)

ctggggtcctaggaccc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6juq A polar filter in DNA polymerases prevents ribonucleotide incorporation.
• Resolution: 2.74 Å
• Binding residue (original residue number in PDB): R38 V40 S42 G60 K217 R240 K241 S242 V243 G244 V245 E246 R247 T248 R273 F295 R330
• Binding residue (residue number reindexed from 1): R38 V40 S42 G60 K217 R240 K241 S242 V243 G244 V245 E246 R247 T248 R273 F295 R330

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0005515 protein binding
• GO:0008296 3'-5'-DNA exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0000731 DNA synthesis involved in DNA repair
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair
• GO:0006974 DNA damage response
• GO:0009432 SOS response
• GO:0042276 error-prone translesion synthesis
• GO:0070987 error-free translesion synthesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:6juq, PDBe:6juq, PDBj:6juq
• PDBsum: 6juq
• PubMed: 31544946
• UniProt: Q47155|DPO4_ECOLI DNA polymerase IV (Gene Name=dinB)