Structure of PDB 6gte Chain A Binding Site BS02

Receptor Information
>6gte Chain A (length=1274) Species: 401614 (Francisella tularensis subsp. novicida U112) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
IYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKAKQ
IIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAK
DTIKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIEL
FKANSDITDIDEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYR
IVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAEELTFDIDYKTSE
VNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGINE
YINLYSQQINDKTLKKYKMSVLFKQILSDTEKLEDDSDVVTTMQSFYEQI
AAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDLSQQVFDD
YSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLSLETIKLA
LEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQISIKYQNQ
GKKDLLQASAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKANILDKDE
HFYLVFEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNFENSTLANGW
DKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGEGYKKIVY
KLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGSPQKGYEK
FEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDEFYREVEN
QGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPNLHTLYWK
ALFDERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANKNKDNPKK
ESVFEYDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEINLLLKEKAN
DVHILSIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTNYHDKLAA
IEKDRDSARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAIVVFQDLN
QVYQKLEKMLIEKLNYLVFKDNEFDKTGGVLRAYQLTAPFETFKKMGKQT
GIIYYVPAGFTSKICPVTGFVNQLYPKYESVSKSQEFFSKFDKICYNLDK
GYFEFSFDYKNFGDKAAKGKWTIASFGSRLINFRNSNWDTREVYPTKELE
KLLKDYSIEYGHGECIKAAICGESDKKFFAKLTSVLNTILQMRNSKTGTE
LDYLISPVADVNGNFFDSRQAPKNMPQDADANGAYHIGLKGLMLLGRIKN
NQEGKKLNLVIKNEEYFEFVQNRN
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB6gte Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
Resolution4.07 Å
Binding residue
(original residue number in PDB)		K131 K132 N185 P196 K341 N612 L661 P663 K671 V672 S675 A676 K677 W734 K823 N825 E827 E1062 T1063 F1064 K1066
Binding residue
(residue number reindexed from 1)		K129 K130 N183 P194 K332 N603 L652 P654 K662 V663 S666 A667 K668 W725 K814 N816 E818 E1041 T1042 F1043 K1045
Enzymatic activity
Enzyme Commision number 3.1.21.1: deoxyribonuclease I.
4.6.1.22: Bacillus subtilis ribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004530 deoxyribonuclease I activity
GO:0016829 lyase activity
Biological Process
GO:0006259 DNA metabolic process
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:6gte, PDBe:6gte, PDBj:6gte
PDBsum6gte
PubMed30503205
UniProtA0Q7Q2|CS12A_FRATN CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)

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