Structure of PDB 5o7t Chain A Binding Site BS02

Receptor Information

>5o7t Chain A (length=540) Species: 271 (Thermus aquaticus)

ALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKAL
RDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVAR
RYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLA
HMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLER
VLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLK
STYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQ
RIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTET
ASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAF
IERYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVRE
AAERKAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAP
KERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE

Ligand information

>5o7t Chain C (length=16)

aaagcgcgccgtggtc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5o7t Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase.
• Resolution: 1.8 Å
• Binding residue (original residue number in PDB): N483 N485 S486 I503 K505 S513 A517 S543 T569 A570 T571 S575 S576 S577 D578 N580 G668 Y671 G672 M673 S674 H676 R677 Q680 R728 R746 N750 Q754
• Binding residue (residue number reindexed from 1): N191 N193 S194 I211 K213 S221 A225 S251 T277 A278 T279 S283 S284 S285 D286 N288 G376 Y379 G380 M381 S382 H384 R385 Q388 R436 R454 N458 Q462

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0001882 nucleoside binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair

External links

• PDB: RCSB:5o7t, PDBe:5o7t, PDBj:5o7t
• PDBsum: 5o7t
• PubMed: 29136072
• UniProt: P19821|DPO1_THEAQ DNA polymerase I, thermostable (Gene Name=polA)