Structure of PDB 5ekg Chain A Binding Site BS02

Receptor Information

>5ekg Chain A (length=425) Species: 10090 (Mus musculus)

GTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGLI
PKFVSFLGKTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLL
ASPHAHISEQAVWALGNIAGDGSAFRDLVIKHGAIDPLLALLAVPDLSTL
ACGYLRNLTWTLSNLCRNKNPAPPLDAVEQILPTLVRLLHHNDPEVLADS
CWAISYLTDGPNERIEMVVKKGVVPQLVKLLGATELPIVTPALRAIGNIV
TGTDEQTQKVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQ
QVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCGI
IEPLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGLD
KIEALQRHENESVYKASLNLIEKYF

Ligand information

>5ekg Chain C (length=9) Species: 32630 (synthetic construct)

GKKRRKLRR

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5ekg Structural and Calorimetric Studies Demonstrate that Xeroderma Pigmentosum Type G (XPG) Can Be Imported to the Nucleus by a Classical Nuclear Import Pathway via a Monopartite NLS Sequence.
• Resolution: 2.8 Å
• Binding residue (original residue number in PDB): S105 R106 P110 W142 N146 A148 S149 G150 T155 Q181 W184 N188 G191 D192 N228 W231
• Binding residue (residue number reindexed from 1): S34 R35 P39 W71 N75 A77 S78 G79 T84 Q110 W113 N117 G120 D121 N157 W160

Enzymatic activity

• Catalytic site (original residue number in PDB): T151 A163 N361 R366 Q367
• Catalytic site (residue number reindexed from 1): T80 A92 N290 R295 Q296

Gene Ontology

Molecular Function

• GO:0061608 nuclear import signal receptor activity

Biological Process

• GO:0006606 protein import into nucleus

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:5ekg, PDBe:5ekg, PDBj:5ekg
• PDBsum: 5ekg
• PubMed: 26812207
• UniProt: P52293|IMA1_MOUSE Importin subunit alpha-1 (Gene Name=Kpna2)