Structure of PDB 5b2p Chain A Binding Site BS02

Receptor Information

>5b2p Chain A (length=1455) Species: 401614 (Francisella tularensis subsp. novicida U112)

MNFKILPIAIDLGVKNTGVFSAFYQKGTSLERLDNKNGKVYELSKDSYTL
LMNNRTARRHQRRGIDRKQLVKRLFKLIWTEQLNLEWDKDTQQAISFLFN
RRGFSFITDGYSVKAILMDIFDDYNGEDDSYLKLATEQESKISEIYNKLM
QKILEFKLMKLCTDIKDDTLKEITSYEFELLADYLANYSESLKTQKFYNI
QEFLKRHATINDRILDTLLTDDLDIWNFNFELHHFVFAVNKIKSEMASGG
RHRSQYFQEITNVLDENNHQEGYLKNFCENLHNKKYSNLSVKNLVNLIGN
LSNLELKPLRKYFNDKIHAKADHWDEQKFTETYCHWILGEWRVGVKDQDK
KDGAKYSYKDLCNELKQKVTKAGLVDFLLELDPCRTIPPYLDNNNRKPPK
CQSLILNPKFLDNQYPNWQQYLQELKKLQSIQNYLDSFETDLKVLKSSKD
QPYFVEYKSSNQQIASGQRDYKDLDARILQFIFDRVKASDELLLNEIYFQ
AKKLKQESSKKLDEVIANSQLSQILKSQHTNGIFEQGTFLHLVCKYYKQR
QRARDSRLYIMPEYRYDKKLHKYNNTGRFDDDNQLLTYCNHKPRQKRYQL
LNDLAGVLQVSPNFLKDKIGSDDDLFISKWLVEHIRGFKKACEDSLKIQK
DNRGLLNHKINIARNTKGKCEKEIFNLICKIEGYKHGLAYELGVLLFGEP
NEASKPEFDRKIKKFNSIYSFAQIQQIAFAERKGNANTCAVCSADNAHRM
QQIKIILSAKAQRLPAIPTRIVDGAVKKMATILAKNIVDDNWQNIKQVLS
AKHQLHIPIITESNAFEFEPALADVKGKSLKDRRKKALERISPENIFKDK
NNRIKEFAEELDHIIPRTLNDEANLICVTGNRIFCLRDNYRSFINLTPQE
QKAFRHALFLADENPIKQAVIRAINNRNRTFVNGTQRYFAEVLANNIYLR
AKKENLNTDKISFDYFGIPTIGNGRGIAEIRQLYEKVDSDIQAYAKGDKP
QASYSHLIDAMLAFCIAADEHRNDGSIGLEIDKNYSLYPDIFSQIKITDN
EFSDKKLVRKKAIEGFNTHRQMTRDGIYAENYLPILIHKELNEVRKGYTW
KNSEEIKIFKGKKYDIQQLNNLVYCLKFVDKPISIDIQISTLEELRNILT
TNNIAATAEYYYINLKTQKLHEYYIENYNTALGYKKYSKEMEFLRSLAYR
SERVKIKSIDDVKQVLDKDSNFIIGKITLPFKKEWQRLYREWQNTTIKDD
YEFLKSFFNVKSITKLHKKVRKDFSLPISTNEGKFLVKRKTWDNNFIYQI
LNDSDSRADGTKPFIPAFDISKNEIVEAIIDSFTSKNIFWLPKNIELQKV
DNKNIFAIDTSKWFEVETPSDLRDIGIATIQYKIDNNSRPKVRVKLDYVI
DDDSKINYFMNHSLLKSRYPDKVLEILKQSTIIEFESSGFNKTIKEMLGM
KLAGI

Ligand information

>5b2p Chain C (length=30)

ccgatatcaccccagcgcacctaatttccc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5b2p Structure and Engineering of Francisella novicida Cas9
• Resolution: 1.7 Å
• Binding residue (original residue number in PDB): F106 K405 Y449 L450 D451 N452 N453 V545 K546 R622 Q677 N725 K789 Y794 G809 N810 M825 S844 A845 Q848 T855 R856 V858 R920 R1047 R1241 D1242 E1449 D1472 R1474 K1583 N1608 K1609
• Binding residue (residue number reindexed from 1): F106 K346 Y390 L391 D392 N393 N394 V486 K487 R554 Q609 N657 K714 Y719 G734 N735 M750 S758 A759 Q762 T769 R770 V772 R834 R891 R1074 D1075 E1282 D1305 R1307 K1416 N1441 K1442

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:5b2p, PDBe:5b2p, PDBj:5b2p
• PDBsum: 5b2p
• PubMed: 26875867
• UniProt: A0Q5Y3|CAS9_FRATN CRISPR-associated endonuclease Cas9 (Gene Name=cas9)