Structure of PDB 4pga Chain A Binding Site BS02

Receptor Information

>4pga Chain A (length=330) Species: 65406 (Pseudomonas sp. 7A)

KLANVVILATGGTIAGAGASAANSATYQAAKVGVDKLIAGVPELADLANV
RGEQVMQIASESITNDDLLKLGKRVAELADSNDVDGIVITHGTDTLEETA
YFLNLVQKTDKPIVVVGSMRPGTAMSADGMLNLYNAVAVASNKDSRGKGV
LVTMNDEIQSGRDVSKSINIKTEAFKSAWGPLGMVVEGKSYWFRLPAKRH
TVNSEFDIKQISSLPQVDIAYSYGNVTDTAYKALAQNGAKALIHAGTGNG
SVSSRVVPALQQLRKNGTQIIRSSHVNQGGFVLRNAEQPDDKNDWVVAHD
LNPEKARILAMVAMTKTQDSKELQRIFWEY

Ligand information

• Ligand ID: NH4
• InChI: InChI=1S/H3N/h1H3/p+1
• InChIKey: QGZKDVFQNNGYKY-UHFFFAOYSA-O
• SMILES:
  ACDLabs 10.04
  CACTVS 3.341
  OpenEye OEToolkits 1.5.0: [NH4+]
• Formula: H4 N
• Name: AMMONIUM ION
• PDB chain: 4pga Chain A Residue 339

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4pga Ion binding induces closed conformation in Pseudomonas 7A glutaminase-asparaginase (PGA): crystal structure of the PGA-SO4(2-)-NH4+ complex at 1.7 A resolution.
• Resolution: 1.7 Å
• Binding residue (original residue number in PDB): E68 D101
• Binding residue (residue number reindexed from 1): E61 D94
• Annotation score: 5

Enzymatic activity

• Catalytic site (original residue number in PDB): T20 Y34 T100 D101 K173 E294
• Catalytic site (residue number reindexed from 1): T13 Y27 T93 D94 K166 E287
• Enzyme Commision number: 3.5.1.38: glutamin-(asparagin-)ase.

Gene Ontology

Molecular Function

• GO:0004067 asparaginase activity
• GO:0004359 glutaminase activity
• GO:0016787 hydrolase activity
• GO:0050417 glutamin-(asparagin-)ase activity

Biological Process

• GO:0006520 amino acid metabolic process
• GO:0006528 asparagine metabolic process

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:4pga, PDBe:4pga, PDBj:4pga
• PDBsum: 4pga
• PubMed: 9020792
• UniProt: P10182|ASPQ_PSES7 Glutaminase-asparaginase (Gene Name=ansB)