Structure of PDB 4dqs Chain A Binding Site BS02

Receptor Information

>4dqs Chain A (length=573) Species: 235909 (Geobacillus kaustophilus HTA426)

KMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFLR
PETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLLA
AYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEHL
VRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVDT
KRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPVL
YSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVRPDTKKVH
TIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESDWLIFAAD
YSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDEVTPNMRR
QAKAVNFGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGVKRYMENI
VQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPIQGSAADI
IKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCRLVPEVME
QAVTLRVPLKVDYHYGSTWYDAK

Ligand information

>4dqs Chain C (length=12)

cgtacgtgatcg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4dqs Structural factors that determine selectivity of a high fidelity DNA polymerase for deoxy-, dideoxy-, and ribonucleotides.
• Resolution: 1.66 Å
• Binding residue (original residue number in PDB): N527 N529 S530 S585 T586 L610 T611 Q612 T613 R615 S617 S618 T619 N622 G711 Y714 S717 G720 L721 R771 F786 R789 Q797
• Binding residue (residue number reindexed from 1): N230 N232 S233 S282 T283 L307 T308 Q309 T310 R312 S314 S315 T316 N319 G408 Y411 S414 G417 L418 R468 F483 R486 Q494

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:4dqs, PDBe:4dqs, PDBj:4dqs
• PDBsum: 4dqs
• PubMed: 22648417
• UniProt: Q5KWC1