Structure of PDB 3sfg Chain A Binding Site BS02

Receptor Information

>3sfg Chain A (length=480) Species: 223997 (Murine norovirus 1)

LPRPSGTYAGLPIADYGDAPPLSTKTMFWRTSPEKLPPGAWEPAYLGSKD
ERVDGPSLQQVMRDQLKPYSEPRGLLPPQEILDAVCDAIENRLENTLEPQ
KPWTFKKACESLDKNTSSGYPYHKQKSKDWTGSAFIGDLGDQATHANNMY
EMGKSMRPIYTAALKDELVKPDKIYGKIKKRLLWGSDLGTMIRAARAFGP
FCDALKETCIFNPIRVGMSMNEDGPFIFARHANFRYHMDADYTRWDSTQQ
RAILKRAGDIMVRLSPEPDLARVVMDDLLAPSLLDVGDYKIVVEEGLPSG
CPCTTQLNSLAHWILTLCAMVEVTRVDPDIVMQESEFSFYGDDEVVSTNL
ELDMVKYTMALRRYGLLPTRADKEEGPLERRQTLQGISFLRRAIVGDQFG
WYGRLDRASIDRQLLWTKGPNHQNPFETLPGHAQRPSQLMALLGEAAMHG
EKYYRTVASRVSKMVVPRHRSVLRWVRFGT

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 3sfg Chain A Residue 623

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3sfg Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase in complex with 2-thiouridine or ribavirin.
• Resolution: 2.209 Å
• Binding residue (original residue number in PDB): D243 D347 E348 S392
• Binding residue (residue number reindexed from 1): D239 D343 E344 S388
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.66: calicivirin.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:3sfg, PDBe:3sfg, PDBj:3sfg
• PDBsum: 3sfg
• PubMed: 22341781
• UniProt: Q80J95|POLG_MNV1 Genome polyprotein (Gene Name=ORF1)