Structure of PDB 3mht Chain A Binding Site BS02

Receptor Information

>3mht Chain A (length=327) Species: 726 (Haemophilus haemolyticus)

MIEIKDKQLTGLRFIDLFAGLGGFRLALESCGAECVYSNEWDKYAQEVYE
MNFGEKPEGDITQVNEKTIPDHDILCAGFPCQAFSISGKQKGFEDSRGTL
FFDIARIVREKKPKVVFMENVKNFASHDNGNTLEVVKNTMNELDYSFHAK
VLNALDYGIPQKRERIYMICFRNDLNIQNFQFPKPFELNTFVKDLLLPDS
EVEHLVIDRKDLVMTNQEIEQTTPKTVRLGIVGKGGQGERIYSTRGIAIT
LSAYGGGIFAKTGGYLVNGKTRKLHPRECARVMGYPDSYKVHPSTSQAYK
QFGNSVVINVLQYIAYNIGSSLNFKPY

Ligand information

>3mht Chain D (length=13)

tgatagcgctatc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3mht Enzymatic C5-cytosine methylation of DNA: mechanistic implications of new crystal structures for HhaL methyltransferase-DNA-AdoHcy complexes.
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): G78 C81 S85 I86 S87 R97 E119 V121 K162 R165 T226 R228 Q237 R240 Y242 I249 T250 S252 A253 Y254 G256 G303 N304
• Binding residue (residue number reindexed from 1): G78 C81 S85 I86 S87 R97 E119 V121 K162 R165 T226 R228 Q237 R240 Y242 I249 T250 S252 A253 Y254 G256 G303 N304

Enzymatic activity

• Catalytic site (original residue number in PDB): C81 E119 R163 R165
• Catalytic site (residue number reindexed from 1): C81 E119 R163 R165
• Enzyme Commision number: 2.1.1.37: DNA (cytosine-5-)-methyltransferase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003886 DNA (cytosine-5-)-methyltransferase activity
• GO:0008168 methyltransferase activity

Biological Process

• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

External links

• PDB: RCSB:3mht, PDBe:3mht, PDBj:3mht
• PDBsum: 3mht
• PubMed: 8800212
• UniProt: P05102|MTH1_HAEPH Type II methyltransferase M.HhaI (Gene Name=hhaIM)