Structure of PDB 3klf Chain A Binding Site BS02

Receptor Information

>3klf Chain A (length=553) Species: 11678 (Human immunodeficiency virus type 1 BH10)

ISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIG
PENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLK
KKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLPQ
GWKGSPAIFQSSMTKILEPFKKQNPDIVIYQYMDDLYVGSDLEIGQHRTK
IEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDS
WTVNDICKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAEL
ELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKT
GKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETW
WTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETK
LGKAGYVTNKGRQKVVPLTNTTNQKTELQAIYLALQDSGLEVNIVTDSQY
ALGIIQAQPDKSESELVNQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKL
VSA

Ligand information

>3klf Chain D (length=20)

cagtccctgttcgggcgcca

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3klf Structural basis of HIV-1 resistance to AZT by excision.
• Resolution: 3.15 Å
• Binding residue (original residue number in PDB): Y183 M184 M230 G231 N255 C258 K259 G262 K263 W266 L289 T473 Q475 Y501
• Binding residue (residue number reindexed from 1): Y182 M183 M229 G230 N254 C257 K258 G261 K262 W265 L288 T472 Q474 Y500

Enzymatic activity

• Enzyme Commision number: 2.7.7.-
  2.7.7.49: RNA-directed DNA polymerase.
  2.7.7.7: DNA-directed DNA polymerase.
  3.1.-.-
  3.1.13.2: exoribonuclease H.
  3.1.26.13: retroviral ribonuclease H.
  3.4.23.16: HIV-1 retropepsin.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003964 RNA-directed DNA polymerase activity
• GO:0004523 RNA-DNA hybrid ribonuclease activity

Biological Process

• GO:0006278 RNA-templated DNA biosynthetic process

External links

• PDB: RCSB:3klf, PDBe:3klf, PDBj:3klf
• PDBsum: 3klf
• PubMed: 20852643
• UniProt: P03366|POL_HV1B1 Gag-Pol polyprotein (Gene Name=gag-pol)