Structure of PDB 2x0x Chain A Binding Site BS02

Receptor Information

>2x0x Chain A (length=728) Species: 83333 (Escherichia coli K-12)

NLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELRSHIQFYDGI
KTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPALY
DHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEG
KYLVQNRVTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTF
KISLPTPIMSGVRTPTRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAG
IGINAGRIRALGSPFHTGCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMW
HLEVESLLVLKNNRGVEGNRVRHMDYGVQINKLMYTRLLKGEDITLFSPS
DVPGLYDAFFADQEEFERLYTKYEKDDSIRKQRVKAVELFSLMMQERAST
GRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLNDVNDENGEI
ALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAMG
RRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELA
KEQGACPWFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTH
GLRNSTLSALMPSETSSQISNATNGIEPPRGYVSIKASKDGILRQVVPDY
EHLHDAYELLWEMPGNDGYLQLVGIMQKFIDQSISANTNYDPSRFPSGKV
PMQQLLKDLLTAYKFGVKTLYYQNTRDG

Ligand information

>2x0x Chain P (length=3) Species: 83333 (Escherichia coli K-12)

YLV

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2x0x Site-Specific Incorporation of 3-Nitrotyrosine as a Probe of Pk(A) Perturbation of Redox-Active Tyrosines in Ribonucleotide Reductase.
• Resolution: 2.3 Å
• Binding residue (original residue number in PDB): A559 K560 Q562 G563 A564 H609 G610
• Binding residue (residue number reindexed from 1): A550 K551 Q553 G554 A555 H600 G601

Enzymatic activity

• Catalytic site (original residue number in PDB): C225 N437 C439 E441 C462 Y730 Y731
• Catalytic site (residue number reindexed from 1): C222 N428 C430 E432 C453 Y721 Y722
• Enzyme Commision number: 1.17.4.1: ribonucleoside-diphosphate reductase.

Gene Ontology

Molecular Function

• GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016491 oxidoreductase activity
• GO:0042802 identical protein binding
• GO:0044183 protein folding chaperone

Biological Process

• GO:0006457 protein folding
• GO:0009185 ribonucleoside diphosphate metabolic process
• GO:0009263 deoxyribonucleotide biosynthetic process
• GO:0009265 2'-deoxyribonucleotide biosynthetic process
• GO:0015949 nucleobase-containing small molecule interconversion

Cellular Component

• GO:0005829 cytosol
• GO:0005971 ribonucleoside-diphosphate reductase complex

External links

• PDB: RCSB:2x0x, PDBe:2x0x, PDBj:2x0x
• PDBsum: 2x0x
• PubMed: 20518462
• UniProt: P00452|RIR1_ECOLI Ribonucleoside-diphosphate reductase 1 subunit alpha (Gene Name=nrdA)