Structure of PDB 2rgr Chain A Binding Site BS02

Receptor Information

>2rgr Chain A (length=721) Species: 4932 (Saccharomyces cerevisiae)

RKSRITNYPKLEDANKAGTKEGYKCTLVLTEGDSALSLAVAGLAVVGRDY
YGCYPLRGKMLNVREASADQILKNAEIQAIKKIMGLQHRKKYEDTKSLRY
GHLMIMTDQDHDGSHIKGLIINFLESSFLGLLDIQGFLLEFITPIIKVSI
TKPTKNTIAFYNMPDYEKWREEESHKFTWKQKYYKGLGTSLAQEVREYFS
NLDRHLKIFHSLQGNDKDYIDLAFSKKKADDRKEWLRQYEPGTVLDPTLK
EIPISDFINKELILFSLADNIRSIPNVLDGFKPGQRKVLYGCFKKNLKSE
LKVAQLAPYVSECTAYHHGEQSLAQTIIGLAQNFVGSNNIYLLLPNGAFG
TRATGGKDAAAARYIYTELNKLTRKIFHPADDPLYKYIQEDEKTVEPEWY
LPILPMILVNGAEGIGTGWSTYIPPFNPLEIIKNIRHLMNDEELEQMHPW
FRGWTGTIEEIEPLRYRMYGRIEQIGDNVLEITELPARTWTSTIKEYLLL
GLSGNDKIKPWIKDMEEQHDDNIKFIITLSPEEMAKTRKIGFYERFKLIS
PISLMNMVAFDPHGKIKKYNSVNEILSEFYYVRLEYYQKRKDHMSERLQW
EVEKYSFQVKFIKMIIEKELTVTNKPRNAIIQELENLGFPRFNKEGKPYY
GSLYGTYEYLLGMRIWSLTKERYQKLLKQKQEKETELENLLKLSAKDIWN
TDLKAFEVGYQEFLQRDAEAR

Ligand information

>2rgr Chain D (length=19)

cgcgcatcgtcatcctcgg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2rgr Structural basis for gate-DNA recognition and bending by type IIA topoisomerases.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): R475 K477 L479 N480 H533 A647 R650 K651 I833 G834 T835 G836 W837 S838 K965
• Binding residue (residue number reindexed from 1): R57 K59 L61 N62 H115 A229 R232 K233 I415 G416 T417 G418 W419 S420 K547

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

External links

• PDB: RCSB:2rgr, PDBe:2rgr, PDBj:2rgr
• PDBsum: 2rgr
• PubMed: 18097402
• UniProt: P06786|TOP2_YEAST DNA topoisomerase 2 (Gene Name=TOP2)