Structure of PDB 2phn Chain A Binding Site BS02

Receptor Information

>2phn Chain A (length=248) Species: 224325 (Archaeoglobus fulgidus DSM 4304)

RVEVFPVEGLPLIKEGDDLAELISSRVRFEDGDVLVVCSTVISKAEGRIR
RLEEFNPSERAKEIAARIGKPAEFVQAVLEESEEVLLDFPFLLVKAKFGN
VCVNAGIDASNVEEGSLLLPPLDPDGSAEKLRRRILELTGKRVGVIITDT
NGRCFRRGVVGFAIGISGVKAMKDWIGRKDLYGRELEVTVECVADEIAAF
ANLLMGEGGDGIPAVVVRGLNVAGEGSMEEIYRSEEEDVIRRCLKRCL

Ligand information

• Ligand ID: MN
• InChI: InChI=1S/Mn/q+2
• InChIKey: WAEMQWOKJMHJLA-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mn+2]
  CACTVS 3.341: [Mn++]
• Formula: Mn
• Name: MANGANESE (II) ION
• DrugBank: DB06757
• PDB chain: 2phn Chain A Residue 2002

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2phn Structure of an Amide Bond Forming F(420):gammagamma-glutamyl Ligase from Archaeoglobus Fulgidus - A Member of a New Family of Non-ribosomal Peptide Synthases.
• Resolution: 1.35 Å
• Binding residue (original residue number in PDB): D109 D150
• Binding residue (residue number reindexed from 1): D108 D149
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 6.3.2.31: coenzyme F420-0:L-glutamate ligase.
  6.3.2.34: coenzyme F420-1:gamma-L-glutamate ligase.

Gene Ontology

Molecular Function

• GO:0005525 GTP binding
• GO:0016874 ligase activity
• GO:0043773 coenzyme F420-0 gamma-glutamyl ligase activity
• GO:0046872 metal ion binding
• GO:0052618 coenzyme F420-0:L-glutamate ligase activity
• GO:0052619 coenzyme F420-1:gamma-L-glutamate ligase activity

Biological Process

• GO:0052645 F420-0 metabolic process

External links

• PDB: RCSB:2phn, PDBe:2phn, PDBj:2phn
• PDBsum: 2phn
• PubMed: 17669425
• UniProt: O28028|COFE_ARCFU Coenzyme F420:L-glutamate ligase (Gene Name=cofE)