Structure of PDB 2hhu Chain A Binding Site BS02

Receptor Information

>2hhu Chain A (length=580) Species: 1422 (Geobacillus stearothermophilus)

KKMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFL
RPETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLL
AAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEH
LVRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVD
TKRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPV
LKKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVR
PDTKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESD
WLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDE
VTPNMRRQAKAVNFGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGV
KRYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPI
QGSAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCR
LVPEVMEQAVTLRVPLKVDYHYGSTWYDAK

Ligand information

>2hhu Chain C (length=13)

cgggctgatcgca

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2hhu The structural basis for the mutagenicity of O6-methyl-guanine lesions.
• Resolution: 1.8 Å
• Binding residue (original residue number in PDB): N527 N529 S530 S585 T586 L610 T611 Q612 T613 R615 S617 S618 T619 E620 N622 G711 Y714 S717 G720 L721 N724 R771 F786 R789 N793 Q797
• Binding residue (residue number reindexed from 1): N231 N233 S234 S289 T290 L314 T315 Q316 T317 R319 S321 S322 T323 E324 N326 G415 Y418 S421 G424 L425 N428 R475 F490 R493 N497 Q501
• Binding affinity: PDBbind-CN: Kd=1.1mM

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:2hhu, PDBe:2hhu, PDBj:2hhu
• PDBsum: 2hhu
• PubMed: 17179038
• UniProt: Q5KWC1