Structure of PDB 1ua0 Chain A Binding Site BS02

Receptor Information

>1ua0 Chain A (length=580) Species: 1422 (Geobacillus stearothermophilus)

AAMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFL
RPETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLL
AAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEH
LVRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVD
TKRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPV
LKKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVR
PDTKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESD
WLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDE
VTPNMRRQAKAVNFGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGV
KRYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPI
QGSAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCR
LVPEVMEQAVTLRVPLKVDYHYGSTWYDAK

Ligand information

>1ua0 Chain C (length=12)

tgcaccatccct

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1ua0 Observing translesion synthesis of an aromatic amine DNA adduct by a high-fidelity DNA polymerase
• Resolution: 2.1 Å
• Binding residue (original residue number in PDB): S530 S585 T586 T611 Q612 T613 S617 S618 T619 E620 N622 Y714 L721 A722 Q723 N724 R771 F786 R789 M790 N793 Q797
• Binding residue (residue number reindexed from 1): S234 S289 T290 T315 Q316 T317 S321 S322 T323 E324 N326 Y418 L425 A426 Q427 N428 R475 F490 R493 M494 N497 Q501

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:1ua0, PDBe:1ua0, PDBj:1ua0
• PDBsum: 1ua0
• PubMed: 15385534
• UniProt: Q5KWC1