Structure of PDB 1u48 Chain A Binding Site BS02

Receptor Information

>1u48 Chain A (length=580) Species: 1422 (Geobacillus stearothermophilus)

AAMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFL
RPETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLL
AAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEH
LVRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVD
TKRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPV
LKKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVR
PDTKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESD
WLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDE
VTPNMRRQAKAVNFGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGV
KRYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPI
QGSAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCR
LVPEVMEQAVTLRVPLKVDYHYGSTWYDAK

Ligand information

>1u48 Chain C (length=13)

atgcgagtcaggc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1u48 Error-prone replication of oxidatively damaged DNA by a high-fidelity DNA polymerase.
• Resolution: 2.1 Å
• Binding residue (original residue number in PDB): N527 N529 S530 S585 T586 L610 T611 S617 S618 T619 N622 A707 G711 Y714 G720 L721 N724 R771 F786 R789
• Binding residue (residue number reindexed from 1): N231 N233 S234 S289 T290 L314 T315 S321 S322 T323 N326 A411 G415 Y418 G424 L425 N428 R475 F490 R493

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:1u48, PDBe:1u48, PDBj:1u48
• PDBsum: 1u48
• PubMed: 15322558
• UniProt: P52026|DPO1_GEOSE DNA polymerase I (Gene Name=polA)