Structure of PDB 1t9j Chain A Binding Site BS02
Receptor Information
>1t9j Chain A (length=151) Species:
3055
(Chlamydomonas reinhardtii) [
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TKYNKEFLLYLAGFVDGDGSIIAQIKPNQSYKFKHQLSLTFQVTEKTQRR
WFLDKLVDEIGVGYVRDRGSVSDYILSEIKPLHNFLTQLQPFLKLKQKQA
NLVLKIIEQLPSAKESPDKFLEVCTWVDQIAALNDSKTRKTTSETVRAVL
D
Ligand information
>1t9j Chain D (length=24) [
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cgaaactgtctcacgacgttttgc
Receptor-Ligand Complex Structure
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PDB
1t9j
Metal-Dependent DNA Cleavage Mechanism of the I-CreI LAGLIDADG Homing Endonuclease.
Resolution
2.0 Å
Binding residue
(original residue number in PDB)
S32 Y33 K34 Q38 Y66 R68 R70 E80 I81 K116 D137 K139
Binding residue
(residue number reindexed from 1)
S30 Y31 K32 Q36 Y64 R66 R68 E78 I79 K114 D135 K137
Binding affinity
PDBbind-CN
: Kd=0.6nM
Enzymatic activity
Catalytic site (original residue number in PDB)
G19 D20
Catalytic site (residue number reindexed from 1)
G17 D18
Enzyme Commision number
3.1.-.-
Gene Ontology
Molecular Function
GO:0004519
endonuclease activity
GO:0042802
identical protein binding
GO:0046872
metal ion binding
Biological Process
GO:0006314
intron homing
Cellular Component
GO:0009507
chloroplast
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Cellular Component
External links
PDB
RCSB:1t9j
,
PDBe:1t9j
,
PDBj:1t9j
PDBsum
1t9j
PubMed
15518550
UniProt
P05725
|DNE1_CHLRE DNA endonuclease I-CreI
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