Structure of PDB 1s9f Chain A Binding Site BS02

Receptor Information

>1s9f Chain A (length=341) Species: 2287 (Saccharolobus solfataricus)

MIVLFVDFDYFYAQVEEVLNPSLKGKPVVVCVFSGRFEDSGAVATANYEA
RKFGVKAGIPIVEAKKILPNAVYLPMRKEVYQQVSSRIMNLLREYSEKIE
IASIDEAYLDISDKVRDYREAYNLGLEIKNKILEKEKITVTVGISKNKVF
AKIAADMAKPNGIKVIDDEEVKRLIRELDIADVPGIGNITAEKLKKLGIN
KLVDTLSIEFDKLKGMIGEAKAKYLISLARDEYNEPIRTRVRKSIGRIVT
MKRNSRNLEEIKPYLFRAIEESYYKLDKRIPKAIHVVAVTEDLDIVSRGR
TFPHGISKETAYSESVKLLQKILEEDERKIRRIGVRFSKFI

Ligand information

>1s9f Chain I (length=17)

tcagtagtccttccccc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1s9f Dpo4 is hindered in extending a G.T mismatch by a reverse wobble
• Resolution: 2.0 Å
• Binding residue (original residue number in PDB): V32 F37 S40 A42 P60 V62 G218 A220 R240 R242 K243 S244 I245 G246 R247 I248 T250 L293 R331 R332 R336
• Binding residue (residue number reindexed from 1): V32 F37 S40 A42 P60 V62 G218 A220 R240 R242 K243 S244 I245 G246 R247 I248 T250 L293 R331 R332 R336

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair
• GO:0006974 DNA damage response
• GO:0042276 error-prone translesion synthesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1s9f, PDBe:1s9f, PDBj:1s9f
• PDBsum: 1s9f
• PubMed: 15077104
• UniProt: Q97W02|DPO4_SACS2 DNA polymerase IV (Gene Name=dbh)