Structure of PDB 1o0t Chain A Binding Site BS02

Receptor Information

>1o0t Chain A (length=455) Species: 76981 (Pseudomonas sp. 'TAC II 18')

GTSSAFTQIDNFSHFYDRGDHLVNGKPSFTVDQVADQLTRSGASWHDLNN
DGVINLTYTFLTAPPVGYASRGLGTFSQFSALQKEQAKLSLESWADVAKV
TFTEGPAARGDDGHMTFANFSASNGGAAFAYLPNSSRKGESWYLINKDYQ
VNKTPGEGNYGRQTLTHEIGHTLGLSHPGDNPTYRDAVYAEDTRAYSVMS
YWSEKNTGQVFTKTGEGAYASAPLLDDIAAVQKLYGANLETRADDTVYGF
NSTADRDFYSATSSTDKLIFSVWDGGGNDTLDFSGFSQNQKINLTAGSFS
DVGGMTGNVSIAQGVTIENAIGGSGNDLLIGNDAANVLKGGAGNDIIYGG
GGADVLWGGTGSDTFVFGAVSDSTPKAADIIKDFQSGFDKIDLTAITKLG
GLNFVDAFTGHAGDAIVSYHQASNAGSLQVDFSGQGVADFLVTTVGQVAT
YDIVA

Ligand information

• Ligand ID: CA
• InChI: InChI=1S/Ca/q+2
• InChIKey: BHPQYMZQTOCNFJ-UHFFFAOYSA-N
• SMILES:
  CACTVS 3.341: [Ca++]
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Ca+2]
• Formula: Ca
• Name: CALCIUM ION
• DrugBank: DB14577
• PDB chain: 1o0t Chain A Residue 701

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1o0t Probing the role of divalent metal ions in a bacterial psychrophilic metalloprotease: binding studies of an enzyme in the crystalline state by x-ray crystallography.
• Resolution: 2.5 Å
• Binding residue (original residue number in PDB): R250 D252 T254 D282 G284 D287
• Binding residue (residue number reindexed from 1): R242 D244 T246 D274 G276 D279
• Annotation score: 4

Enzymatic activity

• Enzyme Commision number: 3.4.24.40: serralysin.

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0005509 calcium ion binding
• GO:0008237 metallopeptidase activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005615 extracellular space
• GO:0031012 extracellular matrix

External links

• PDB: RCSB:1o0t, PDBe:1o0t, PDBj:1o0t
• PDBsum: 1o0t
• PubMed: 12837794
• UniProt: O69771