Structure of PDB 1m0e Chain A Binding Site BS02

Receptor Information

>1m0e Chain A (length=327) Species: 726 (Haemophilus haemolyticus)

MIEIKDKQLTGLRFIDLFAGLGGFRLALESCGAECVYSNEWDKYAQEVYE
MNFGEKPEGDITQVNEKTIPDHDILCAGFPCQAFSISGKQKGFEDSRGTL
FFDIARIVREKKPKVVFMENVKNFASHDNGNTLEVVKNTMNELDYSFHAK
VLNALDYGIPQKRERIYMICFRNDLNIQNFQFPKPFELNTFVKDLLLPDS
EVEHLVIDRKDLVMTNQEIEQTTPKTVRLGIVGKGGQGERIYSTRGIAIT
LSAYGGGIFAKTGGYLVNGKTRKLHPRECARVMGYPDSYKVHPSTSQAYK
QFGNSVVINVLQYIAYNIGSSLNFKPY

Ligand information

>1m0e Chain D (length=12)

gtcagcgcatgg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1m0e ZEBULARINE: A NOVEL DNA METHYLATION INHIBITOR THAT FORMS A COVALENT COMPLEX WITH DNA METHYLTRANSFERASES
• Resolution: 2.5 Å
• Binding residue (original residue number in PDB): F79 C81 S85 I86 S87 G88 K89 R97 E119 V121 K162 R163 R165 R228 Q237 R240 Y242 I249 T250 S252 A253 Y254 G256 G303 N304
• Binding residue (residue number reindexed from 1): F79 C81 S85 I86 S87 G88 K89 R97 E119 V121 K162 R163 R165 R228 Q237 R240 Y242 I249 T250 S252 A253 Y254 G256 G303 N304

Enzymatic activity

• Catalytic site (original residue number in PDB): C81 E119 R163 R165
• Catalytic site (residue number reindexed from 1): C81 E119 R163 R165
• Enzyme Commision number: 2.1.1.37: DNA (cytosine-5-)-methyltransferase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003886 DNA (cytosine-5-)-methyltransferase activity
• GO:0008168 methyltransferase activity

Biological Process

• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

External links

• PDB: RCSB:1m0e, PDBe:1m0e, PDBj:1m0e
• PDBsum: 1m0e
• PubMed: 12206775
• UniProt: P05102|MTH1_HAEPH Type II methyltransferase M.HhaI (Gene Name=hhaIM)