Structure of PDB 1lwt Chain A Binding Site BS02

Receptor Information

>1lwt Chain A (length=448) Species: 4932 (Saccharomyces cerevisiae)

CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSV
VQKSQHRAHKSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYF
EVITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNK
AYFEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTI
EGPKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAEY
KDRKAKTVNLYSKVVRGNGIRNNLNTENPLWDAIVGLGFLKDGVKNIPSF
LSTDNIGTRETFLAGLIDSDGYVTDEHGIKATIKTIHTSVRDGLVSLARS
LGLVVSVNAEPAKVDMNGTKHKISYAIYMSGGDVLLNVLSKCAGSKKFRP
APAAAFARECRGFYFELQELKEDDYYGITLSDDSDHQFLLANQVVVHN

Ligand information

>1lwt Chain C (length=37)

gccatttcattacctctttctccgcacccgacataga

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1lwt Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): Q55 H56 R57 V126 S127 K128 S129 G168 S169 H170 S227 A261 N274 R277 K336 S362 N364 E366 H377 Y384
• Binding residue (residue number reindexed from 1): Q55 H56 R57 V124 S125 K126 S127 G166 S167 H168 S225 A255 N268 R271 K330 S356 N358 E360 H371 Y378

Enzymatic activity

• Catalytic site (original residue number in PDB): C1 N76 T78 H79 K301 K403 G433 I434 N454
• Catalytic site (residue number reindexed from 1): C1 N74 T76 H77 K295 K397 G427 I428 N448
• Enzyme Commision number: 3.1.-.-
  7.1.2.2: H(+)-transporting two-sector ATPase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0004519 endonuclease activity

Biological Process

• GO:0016539 intein-mediated protein splicing
• GO:0030908 protein splicing

External links

• PDB: RCSB:1lwt, PDBe:1lwt, PDBj:1lwt
• PDBsum: 1lwt
• PubMed: 12219083
• UniProt: P17255|VATA_YEAST V-type proton ATPase catalytic subunit A (Gene Name=VMA1)