Structure of PDB 1lws Chain A Binding Site BS02

Receptor Information

>1lws Chain A (length=427) Species: 4932 (Saccharomyces cerevisiae)

CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRETMYSV
VQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVF
EVITFEMGQKKAPDGRIVELVKEVSKSYPISPERANELVESYRKASNKAY
FEWTIEARDLSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEG
PKVLAYLLGLWIGDGLSDRATFSVDSRDTSLMERVTEYAEKLNLCAVAKT
VNLYSENPLWDAIVGLGFLKDGVKNIPSFLSTDNIGTRETFLAGLIDSDG
YVTDEHGIKATIKTIHTSVRDGLVSLARSLGLVVSVNAEPAKVDMNGTKH
KISYAIYMSGGDVLLNVLSKCAGSKKFRPAPAAAFARECRGFYFELQELK
EDDYYGITLSDDSDHQFLLANQVVVHN

Ligand information

>1lws Chain C (length=34)

atttcattacctctttctccgcacccgacataga

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1lws Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): Q55 H56 R57 A58 R65 K124 S127 K128 S129 S169 H170 S227 V260 A261 H333 S362 N364 E366 N373 H377 Y384
• Binding residue (residue number reindexed from 1): Q55 H56 R57 A58 R65 K122 S125 K126 S127 S165 H166 S223 V247 A248 H306 S335 N337 E339 N346 H350 Y357

Enzymatic activity

• Catalytic site (original residue number in PDB): C1 N76 T78 H79 K301 K403 G433 I434 N454
• Catalytic site (residue number reindexed from 1): C1 N76 T78 H79 K274 K376 G406 I407 N427
• Enzyme Commision number: 3.1.-.-
  7.1.2.2: H(+)-transporting two-sector ATPase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0004519 endonuclease activity

Biological Process

• GO:0016539 intein-mediated protein splicing
• GO:0030908 protein splicing

External links

• PDB: RCSB:1lws, PDBe:1lws, PDBj:1lws
• PDBsum: 1lws
• PubMed: 12219083
• UniProt: P17255|VATA_YEAST V-type proton ATPase catalytic subunit A (Gene Name=VMA1)