Structure of PDB 1j2b Chain A Binding Site BS02

Receptor Information
>1j2b Chain A (length=576) Species: 53953 (Pyrococcus horikoshii) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MLKFEIKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQMVVEPKELEKMG
FEIIITNSYIIYKDEELRRKALELGIHRMLDYNGIIEVDSGSFQLMKYGS
IEVSNREIIEFQHRIGVDIGTFLDIPTPPDAPREQAVKELEITLSRAREA
EEIKEIPMNATIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLESYRF
RDVVDIVISSKMALRPDRPVHLFGAGHPIVFALAVAMGVDLFDSASYALY
AKDDRYMTPEGTKRLDELDYFPCSCPVCSKYTPQELREMPKEERTRLLAL
HNLWVIKEEIKRVKQAIKEGELWRLVDERARSHPKLYSAYKRLLEHYTFL
EEFEPITKKSALFKISNESLRWPVVRRAKERAKSINERFGELVEHPIFGR
VSRYLSLTYPFAQSEAEDDFKIEKPTKEDAIKYVMAIAEYQFGEGASRAF
DDAKVELSKTGMPRQVKVNGKRLATVRADDGLLTLGIEGAKRLHRVLPYP
RMRVVVNKEAEPFARKGKDVFAKFVIFADPGIRPYDEVLVVNENDELLAT
GQALLSGREMIVFQYGRAVKVRKGVE
Ligand information
>1j2b Chain D (length=71) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gggcccguggucuaguugacgccgcccuuacgaggcggagguccgggguu
caaguccccgcgggcccacca
<<<<<<...........<<<.<<<<<.......>>>>>..>>><<<<<..
.....>>>>>.>>>>>>....
Receptor-Ligand Complex Structure
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PDB1j2b Alternative Tertiary Structure of tRNA for Recognition by a Posttranscriptional Modification Enzyme
Resolution3.3 Å
Binding residue
(original residue number in PDB)		N41 K43 Y65 I66 D95 S98 F99 Q100 Y104 D130 P132 D136 Y173 V198 L201 E202 Y204 R205 F206 D208 F229 G230 A231 G232 H233 D249 S250 A251 S252 L255 Y256 R261 T268 H339
Binding residue
(residue number reindexed from 1)		N35 K37 Y59 I60 D89 S92 F93 Q94 Y98 D124 P126 D130 Y167 V192 L195 E196 Y198 R199 F200 D202 F223 G224 A225 G226 H227 D243 S244 A245 S246 L249 Y250 R255 T262 H333
Enzymatic activity
Catalytic site (original residue number in PDB) D249 C279 C281 C284 H307
Catalytic site (residue number reindexed from 1) D243 C273 C275 C278 H301
Enzyme Commision number 2.4.2.48: tRNA-guanine(15) transglycosylase.
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0008270 zinc ion binding
GO:0016757 glycosyltransferase activity
GO:0016763 pentosyltransferase activity
GO:0046872 metal ion binding
Biological Process
GO:0002099 tRNA wobble guanine modification
GO:0006400 tRNA modification
GO:0008033 tRNA processing
GO:0008616 queuosine biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1j2b, PDBe:1j2b, PDBj:1j2b
PDBsum1j2b
PubMed12732145
UniProtO58843|ATGT_PYRHO tRNA-guanine(15) transglycosylase (Gene Name=tgtA)

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