Structure of PDB 1gcz Chain A Binding Site BS02

PDB	1gcz Coumarin and chromen-4-one analogues as tautomerase inhibitors of macrophage migration inhibitory factor: discovery and X-ray crystallography.
Resolution	1.9 Å
Binding residue (original residue number in PDB)	Y95 N97
Binding residue (residue number reindexed from 1)	Y95 N97
Annotation score	1
Binding affinity	MOAD: Ki=7.4uM PDBbind-CN: -logKd/Ki=5.13,Ki=7.4uM BindingDB: Ki=7400nM

Enzymatic activity

Enzyme Commision number

5.3.2.1: phenylpyruvate tautomerase.
5.3.3.12: L-dopachrome isomerase.

Gene Ontology

	Molecular Function
GO:0002020	protease binding
GO:0004167	dopachrome isomerase activity
GO:0005125	cytokine activity
GO:0005126	cytokine receptor binding
GO:0005515	protein binding
GO:0016853	isomerase activity
GO:0042056	chemoattractant activity
GO:0042802	identical protein binding
GO:0050178	phenylpyruvate tautomerase activity

	Biological Process
GO:0001516	prostaglandin biosynthetic process
GO:0001819	positive regulation of cytokine production
GO:0001934	positive regulation of protein phosphorylation
GO:0002906	negative regulation of mature B cell apoptotic process
GO:0006954	inflammatory response
GO:0007166	cell surface receptor signaling pathway
GO:0008284	positive regulation of cell population proliferation
GO:0010629	negative regulation of gene expression
GO:0010760	negative regulation of macrophage chemotaxis
GO:0019752	carboxylic acid metabolic process
GO:0030330	DNA damage response, signal transduction by p53 class mediator
GO:0030336	negative regulation of cell migration
GO:0030890	positive regulation of B cell proliferation
GO:0031666	positive regulation of lipopolysaccharide-mediated signaling pathway
GO:0032760	positive regulation of tumor necrosis factor production
GO:0033033	negative regulation of myeloid cell apoptotic process
GO:0042127	regulation of cell population proliferation
GO:0042327	positive regulation of phosphorylation
GO:0043030	regulation of macrophage activation
GO:0043066	negative regulation of apoptotic process
GO:0043406	positive regulation of MAP kinase activity
GO:0043518	negative regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045087	innate immune response
GO:0048146	positive regulation of fibroblast proliferation
GO:0050918	positive chemotaxis
GO:0051248	negative regulation of protein metabolic process
GO:0061078	positive regulation of prostaglandin secretion involved in immune response
GO:0061081	positive regulation of myeloid leukocyte cytokine production involved in immune response
GO:0070207	protein homotrimerization
GO:0070374	positive regulation of ERK1 and ERK2 cascade
GO:0090238	positive regulation of arachidonate secretion
GO:0090398	cellular senescence
GO:0141163	positive regulation of cAMP/PKA signal transduction
GO:1902166	negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
GO:2000343	positive regulation of chemokine (C-X-C motif) ligand 2 production
GO:2000773	negative regulation of cellular senescence

	Cellular Component
GO:0005576	extracellular region
GO:0005615	extracellular space
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0009986	cell surface
GO:0031982	vesicle
GO:0034774	secretory granule lumen
GO:0070062	extracellular exosome
GO:1904813	ficolin-1-rich granule lumen

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:1gcz, PDBe:1gcz, PDBj:1gcz
PDBsum	1gcz
PubMed	11170644
UniProt	P14174\|MIF_HUMAN Macrophage migration inhibitory factor (Gene Name=MIF)

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