Structure of PDB 1g15 Chain A Binding Site BS02

Receptor Information

>1g15 Chain A (length=141) Species: 562 (Escherichia coli)

KQVEIFTDGSALGNPGPGGYGAILRYRGREKTFSAGYTRTTNNRMELMAA
IVALEALKEHAEVILSTDSQYVRQGITQWIHNWKARGWKPVKNVDLWQRL
DAALGQHQIKWEWVGHPENERADELARAAAMNPTLEDTGYQ

Ligand information

• Ligand ID: MN
• InChI: InChI=1S/Mn/q+2
• InChIKey: WAEMQWOKJMHJLA-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mn+2]
  CACTVS 3.341: [Mn++]
• Formula: Mn
• Name: MANGANESE (II) ION
• DrugBank: DB06757
• PDB chain: 1g15 Chain A Residue 202

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1g15 Co-crystal of Escherichia coli RNase HI with Mn2+ ions reveals two divalent metals bound in the active site.
• Resolution: 1.9 Å
• Binding residue (original residue number in PDB): D10 D134
• Binding residue (residue number reindexed from 1): D8 D123
• Annotation score: 4

Enzymatic activity

• Catalytic site (original residue number in PDB): D10 G11 E48 D70 D134
• Catalytic site (residue number reindexed from 1): D8 G9 E46 D68 D123
• Enzyme Commision number: 3.1.26.4: ribonuclease H.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003676 nucleic acid binding
• GO:0004519 endonuclease activity
• GO:0004523 RNA-DNA hybrid ribonuclease activity
• GO:0005515 protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006401 RNA catabolic process
• GO:0043137 DNA replication, removal of RNA primer

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1g15, PDBe:1g15, PDBj:1g15
• PDBsum: 1g15
• PubMed: 11083878
• UniProt: P0A7Y4|RNH_ECOLI Ribonuclease HI (Gene Name=rnhA)