Structure of PDB 1flo Chain A Binding Site BS02

Receptor Information

>1flo Chain A (length=405) Species: 4932 (Saccharomyces cerevisiae)

PQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMITHNGTA
IKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWE
FTIIPYYSDITDIVSSLQLQFESKGNSHSKKMLKALLSEGESIWEITEKI
LNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYL
GVIIQCLVTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNR
TGNSSSNKQEYQLLKDNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLM
TSFLSMKGLTELTNVVGNWSDKTTYTHQITAIPDHYFALVSRYYAYDPIS
KEMIALKDETNPIEEWQHIEQLKGSAEGSIRYPAWNGIISQEVLDYLSSY
INRRI

Ligand information

>1flo Chain F (length=20)

tttaaaagaataggaacttc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1flo Crystal structure of a Flp recombinase-Holliday junction complex: assembly of an active oligomer by helix swapping.
• Resolution: 2.65 Å
• Binding residue (original residue number in PDB): H47 K53 A55 T56 Y60 T83 Q84 F171 F192 S193 K223 R281 N284 N300 K303 S304 H305
• Binding residue (residue number reindexed from 1): H46 K52 A54 T55 Y59 T82 Q83 F160 F181 S182 K212 R270 N273 N289 K292 S293 H294

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003690 double-stranded DNA binding
• GO:0003697 single-stranded DNA binding
• GO:0008301 DNA binding, bending
• GO:0009009 site-specific recombinase activity

Biological Process

• GO:0006310 DNA recombination
• GO:0015074 DNA integration
• GO:0042150 plasmid recombination

Cellular Component

• GO:0005575 cellular_component

External links

• PDB: RCSB:1flo, PDBe:1flo, PDBj:1flo
• PDBsum: 1flo
• PubMed: 11090626
• UniProt: P03870|FLP_YEAST Site-specific recombinase Flp (Gene Name=FLP1)