Structure of PDB 1f5a Chain A Binding Site BS02

Receptor Information

>1f5a Chain A (length=463) Species: 9913 (Bos taurus)

YGITSPISLAAPKETDCLLTQKLVETLKPFGVFEEEEELQRRILILGKLN
NLVKEWIREISESKNLPQSVIENVGGKIFTFGSYRLGVHTKGADIDALCV
APRHVDRSDFFTSFYDKLKLQEEVKDLRAVEEAFVPVIKLCFDGIEIDIL
FARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDEILHLVPNIDN
FRLTLRAIKLWAKRHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLV
HKFFLVFSKWEWPNPVLLKQPEECNLNLPVWDPRVNPSDRYHLMPIITPA
YPQQNSTYNVSVSTRMVMVEEFKQGLAITDEILLSKAEWSKLFEAPNFFQ
KYKHYIVLLASAPTEKQRLEWVGLVESKIRILVGSLEKNEFITLAHVNPQ
SFPAKEEFRTMWVIGLVFKDLTYDIQSFTDTVYRQAINSKMFEVDMKIAA
MHVKRKQLHQLLP

Ligand information

• Ligand ID: MN
• InChI: InChI=1S/Mn/q+2
• InChIKey: WAEMQWOKJMHJLA-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mn+2]
  CACTVS 3.341: [Mn++]
• Formula: Mn
• Name: MANGANESE (II) ION
• DrugBank: DB06757
• PDB chain: 1f5a Chain A Residue 1002

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1f5a Crystal structure of mammalian poly(A) polymerase in complex with an analog of ATP.
• Resolution: 2.5 Å
• Binding residue (original residue number in PDB): D113 D115
• Binding residue (residue number reindexed from 1): D94 D96
• Annotation score: 1

Enzymatic activity

• Catalytic site (original residue number in PDB): S102 D113 D115 D167 K228 Y237
• Catalytic site (residue number reindexed from 1): S83 D94 D96 D148 K209 Y218
• Enzyme Commision number: 2.7.7.19: polynucleotide adenylyltransferase.

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0016779 nucleotidyltransferase activity
• GO:1990817 poly(A) RNA polymerase activity

Biological Process

• GO:0031123 RNA 3'-end processing

Cellular Component

• GO:0005634 nucleus

External links

• PDB: RCSB:1f5a, PDBe:1f5a, PDBj:1f5a
• PDBsum: 1f5a
• PubMed: 10944102
• UniProt: P25500|PAPOA_BOVIN Poly(A) polymerase alpha (Gene Name=PAPOLA)