Structure of PDB 1bsu Chain A Binding Site BS02

Receptor Information
>1bsu Chain A (length=236) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVKTYNINELNE
IPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDS
EDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGR
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
PDB1bsu Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease.
Resolution2.0 Å
Binding residue
(original residue number in PDB)		Q69 N70 S183 G184 N185 S223
Binding residue
(residue number reindexed from 1)		Q68 N69 S175 G176 N177 S215
Binding affinityPDBbind-CN: Kd=230nM
Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:1bsu, PDBe:1bsu, PDBj:1bsu
PDBsum1bsu
PubMed10074946
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

[Back to BioLiP]