Structure of PDB 8cen Chain 7 Binding Site BS02

Receptor Information
>8cen Chain 7 (length=615) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
PADFVPDSVSGMFRSHDFSYLRLRPDHASRPLWISPSDGRIILESFSPLA
EQAQDFLVTIAEPISRPSHIHEYKITAYSLYAAVSVGLETDDIISVLDRL
SKVPVAESIINFIKGATISYGKVKLVIKHNRYFVETTQADILQMLLNDSV
IGVHSFEIANESVEVVKKRCQEIDYPVLEEYDFRNDHRNPDLDIDLKPST
QIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIKKSVIVL
CTSSVSVMQWRQQFLQWCTLQPENCAVFTSDNKEMFQTESGLVVSTYSMV
ANTRNRSHDSQKVMDFLTGREWGFIILDEVHVVPAAMFRRVVSTIAAHAK
LGLTATLVREDDKIGDLNFLIGPKLYEANWMELSQKGHIANVQCAEVWCP
MTAEFYQEYLRETARKRMLLYIMNPTKFQACQFLIQYHERRGDKIIVFSD
NVYALQEYALKMGKPFIYGSTPQQERMNILQNFQYNDQINTIFLSKVGDT
SIDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVS
KDTQEMYYSTKRQAFLVDQGYAFKVITHLHGMENIPNLAYASPRERRELL
QEVLLKNHPLIRKMY
Ligand information
>8cen Chain T (length=73) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
atatttttatgtatgtacaacacacatcggaggtgaatcgaacgttccat
agctattatatacacagcgtgct
Receptor-Ligand Complex Structure
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PDB8cen Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain.
Resolution3.0 Å
Binding residue
(original residue number in PDB)		S413 S440 K443 T456 S458 M459 R464 S470 N611 V612 G629 S655 K656 V657 H676
Binding residue
(residue number reindexed from 1)		S253 S280 K283 T296 S298 M299 R304 S310 N451 V452 G469 S495 K496 V497 H516
Enzymatic activity
Enzyme Commision number 5.6.2.4: DNA 3'-5' helicase.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0004386 helicase activity
GO:0005524 ATP binding
GO:0015616 DNA translocase activity
GO:0016787 hydrolase activity
GO:0016853 isomerase activity
GO:0016887 ATP hydrolysis activity
GO:0043138 3'-5' DNA helicase activity
Biological Process
GO:0000019 regulation of mitotic recombination
GO:0001111 RNA polymerase II promoter clearance
GO:0001113 transcription open complex formation at RNA polymerase II promoter
GO:0001174 transcriptional start site selection at RNA polymerase II promoter
GO:0006281 DNA repair
GO:0006289 nucleotide-excision repair
GO:0006366 transcription by RNA polymerase II
GO:0006367 transcription initiation at RNA polymerase II promoter
GO:0016973 poly(A)+ mRNA export from nucleus
GO:0032508 DNA duplex unwinding
Cellular Component
GO:0000112 nucleotide-excision repair factor 3 complex
GO:0000439 transcription factor TFIIH core complex
GO:0005634 nucleus
GO:0005675 transcription factor TFIIH holo complex
GO:0005829 cytosol
GO:0097550 transcription preinitiation complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8cen, PDBe:8cen, PDBj:8cen
PDBsum8cen
PubMed37014863
UniProtQ00578|RAD25_YEAST General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2 (Gene Name=SSL2)

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