Structure of PDB 7d5s Chain 5J Binding Site BS02

Receptor Information

>7d5s Chain 5J (length=151) Species: 559292 (Saccharomyces cerevisiae S288C)

SKFQEIETNLKKLPKLETGFDALANKKKKKNVLPSNDWFTLPKPDDNMRR
EVQRDLLLIKHRAALDPKRHYKKQRWEVPERFAIGTIIEDKSEFYSSRMN
RKERKSTILETLMGDEASNKYFKRKYNEIQEKSTSGRKAHYKKMKEMRKK
R

Ligand information

>7d5s Chain SA (length=859)

aagauaguuaucugguugauccugccaguagucauaugcuugucucacaa
auuagugacaauaaauaacgauacagggcccauucgggucuuguaauugg
aaugaguacaauguaaauaccuuaacgaggaacaauuggagggcaagucu
ggugccagcagccgcgguaauuccagcuccaauagcguauauuaaaguug
uugcaggcucguaguugaacuuagggacggucgggggcaucaguauucaa
uugucagaggugaaauucuuggauuuauugaagacuaacuacugcgaaag
cauuugccaaggacguuuucauuaaucaagaacgaaaguuaggggaucga
agaugaucagauaccgucguagucuuaacuggggggaguauggucgcaag
gcugaaacuuaaaggaauugacggaagggcaccaccaggaguggagccug
cggcucaacacggggaaacucaccagguccagacacaauaaggauugaca
uucuugauuuugugggugguggugcauugaugcccuuguucugggccacg
cgcgcuacacugacggagccagcgagucuaaccuuggccgagaggucuug
guaaucuugugaaacuccgucgugcuggggauauucaacgaggaauuccu
aguaagcgcaagucaucagcuugcguugauuacgucccugcccuuuguac
acaccgcccgucgcuaguaccgauugaauggcuuagugaggccucaggau
cugcuuagagaagggggcaacuccaucucagagcggagaauuuggacaaa
cuuggucauuuagaggaacuaaaagucguaacaagguuuccguaggugaa
ccugcggaa

............................<<<<.<<<<<<...........
..................<<<<<<<.<<<<<....>>>>>.>>>.>>>>.
.....<<..<...........>..>>.........<<<<<<.......<<
<....>>>..................>>>>>>..>>>>>>>>>>......
...<..<.<<...<<<.......<<<<<<.<<...<<<<..<<..<<<<<
<.<...<<<......>>>......>.>>>>>>..>>.......<<....>
>...>>>>..>.>>>>.>>>...>>>...>>.>......<<<<<...<..
.<<<<.........>>>>...>>>>>>...>...................
.<<.........>>.....<<<<<<<<<<<<..<<.<<<<<<..<<<.<<
<<.......................<<.<....<<<<<........<...
..>......>>>>>......<<<........>>>...>.>>.......>>
>>.>>>.....<<<<<<<............<<<..<<<<....>>>>..>
>>............>>>>>>>.......................>>>>>>
....<<<<<<<<.......>>>>>>>>......>>...>>>>>>>.....
.......>>>>>..<<<..<<....<.<<<..<.<<<..<.<<......<
<<<<<<.<<<<.<<<<....>>>>.>>>>.>>>>>>>......>>.>..>
>>.>..>>>>....>>..>>>................<<<<<<<<<....
>>>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7d5s Cryo-EM structure of 90S preribosome with inactive Utp24 (state A2)
• Resolution: 4.6 Å
• Binding residue (original residue number in PDB): P132 K133 H135 Y136 K137 K138 R189 K190 G201 R202 Y206 K210 R213
• Binding residue (residue number reindexed from 1): P67 K68 H70 Y71 K72 K73 R124 K125 G136 R137 Y141 K145 R148

Gene Ontology

Molecular Function

• GO:0003674 molecular_function

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0006396 RNA processing
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus

External links

• PDB: RCSB:7d5s, PDBe:7d5s, PDBj:7d5s
• PDBsum: 7d5s
• UniProt: Q12035|FCF2_YEAST rRNA-processing protein FCF2 (Gene Name=FCF2)