Structure of PDB 8h6e Chain 5B Binding Site BS02

Receptor Information
>8h6e Chain 5B (length=2214) Species: 9606 (Homo sapiens) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MSEEKLQEKARKWQQLQAKRYAEKREDMPPEHVRKIIRDHDKRVYLGALK
YMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQ
WGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQL
ELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLAN
QLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDW
NEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTE
DPDLPAFYFDPLINPISHREPLPDDDEEFELPEFVEPFLKDTPLYTDNTA
NGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQK
LLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQ
GYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCRE
VLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLM
RQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLE
RWLGNLLARQFEGRSHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLS
EAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRG
ATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTV
HWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREEL
GLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPL
EKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQD
VWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNN
VVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGP
PQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYL
TEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRS
VTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVW
NLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWN
TALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPV
VFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSQLIPNLYRY
IQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINT
LFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYR
TDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNA
QRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKI
SLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYK
MNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDY
DSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPG
SKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGEL
FSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKI
IHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDP
LEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYD
DWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTL
TDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEIFSSKT
EWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLR
AQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKE
MEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPG
SCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFF
MVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQ
EGEVYSADREDLYA
Ligand information
>8h6e Chain 6A (length=59) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
acgauacagagaagauuagcauggccccugcgcaaggaugacauucguga
agcguauuu
..................................................
.........
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8h6e Structural Insights into Human Exon-defined Spliceosome Prior to Activation
Resolution3.2 Å
Binding residue
(original residue number in PDB)		D1556 L1557 I1574
Binding residue
(residue number reindexed from 1)		D1485 L1486 I1503
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0017070 U6 snRNA binding
GO:0030619 U1 snRNA binding
GO:0030620 U2 snRNA binding
GO:0030623 U5 snRNA binding
GO:0070530 K63-linked polyubiquitin modification-dependent protein binding
GO:0097157 pre-mRNA intronic binding
GO:0140492 metal-dependent deubiquitinase activity
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000375 RNA splicing, via transesterification reactions
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0071222 cellular response to lipopolysaccharide
GO:0071356 cellular response to tumor necrosis factor
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0016020 membrane
GO:0016607 nuclear speck
GO:0030532 small nuclear ribonucleoprotein complex
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071005 U2-type precatalytic spliceosome
GO:0071006 U2-type catalytic step 1 spliceosome
GO:0071007 U2-type catalytic step 2 spliceosome
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8h6e, PDBe:8h6e, PDBj:8h6e
PDBsum8h6e
PubMed38658629
UniProtQ6P2Q9|PRP8_HUMAN Pre-mRNA-processing-splicing factor 8 (Gene Name=PRPF8)

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