Structure of PDB 4wzo Chain 49 Binding Site BS02

Receptor Information

>4wzo Chain 49 (length=181) Species: 300852 (Thermus thermophilus HB8)

PLDVALKRKYYEEVRPELIRRFGYQNVWEVPRLEKVVINQGLGEAKEDAR
ILEKAAQELALITGQKPAVTRAKKSISNFKLRKGMPIGLRVTLRRDRMWI
FLEKLLNVALPRIRDFRGLNPNSFDGRGNYNLGLREQLIFPEITYDMVDA
LRGMDIAVVTTAETDEEARALLELLGFPFRK

Ligand information

>4wzo Chain 14 (length=2877)

aagaugguaagggcccacgguggaugccucggcacccgagccgaugaagg
acguggcuaccugcgauaagccagggggagccgguagcgggcguggaucc
cuggauguccgaaugggggaacccggccggcgggaacgccggucaccgcg
cuugcgcggggggaaccuggggaacugaaacaucucaguacccagaggag
aggaaagagaaaucgacucccugaguagcggcgagcgaaaggggaccagc
cuaaaccguccggcuuguccgggcggggucguggggcccucggacaccga
auccccagccuagccgaagcuguugggaagcagcgccagagagggugaaa
gccccguaggcgaaagguggggggauaggugaggguacccgaguaccccg
ugguucguggagccaugggggaaucugggcggaccaccgccuaaggcuaa
guacuccgggugaccgauagcgcaccaguaccgugagggaaaggugaaaa
gaaccccgggaggggagugaaauagagccugaaaccgugggcuuacaagc
agucacggccccgcaagggguuguggcgugccuauugaagcaugagccgg
cgacucacggucgugggcgagcuuaagccguugaggcggaggcguaggga
aaccgaguccgaacagggcgcaaacggcccgcacgcgggccgaaaagucc
gcggccguggacccgaaaccgggcgagcuagcccuggccagggugaagcu
ggggugagacccaguggaggcccgaaccggugggggaugcaaaccccucg
gaugagcuggggcuaggagugaaaagcuaaccgagcccggagauagcugg
uucuccccgaaaugacuuuagggucagccucaggcgcugacuggggccug
uagagcacugauagggcuagggggcccaccagccuaccaaacccugucaa
acuccgaagggucccagguggagccugggagugagggcgcgagcgauaac
guccgcguccgagcgcgggaacaaccgagaccgccagcuaaggcccccaa
gucugggcuaagugguaaaggauguggcgccgcgaagacagccaggaugu
uggcuuagaagcagccugcguaauagcucacuggucgaguggcgccgcgc
cgaaaaugaucggggcuuaagcccagcgccgaagcugcgggucuggggga
ugaccccaggcgguaggggagcguucccgaugccgaugaaggccgacccg
cgagggcggcuggagguaagggaagugcgaaugccggcaugaguaacgau
aaagagggugagaaucccucucgccguaagcccaaggguuccuacgcaau
ggucgucagcguaggguuaggcgggaccuaaggugaagccgaaaggcgua
gccgaagggcagccgguuaauauuccggcccuucccgcaggugcgauggg
gggacgcucuaggcuagggggaccggagccauggacgagcccggccagaa
gcgcagggugggagguaggcaaauccgccucccaacaagcucugcguggu
ggggaagcccguacgggugacaaccccccgaagccagggagccaagaaaa
gccucuaagcacaaccugcgggaacccguaccgcaaaccgacacaggugg
gcgggugcaagagcacucaggcgcgcgggagaacccucgccaaggaacuc
ugcaaguuggccccguaacuucgggagaaggggugcucccuggggugaug
agccccggggagccgcagugaacaggcucuggcgacuguuuaccaaaaac
acagcucucugcgaacucguaagaggagguauagggagcgacgcuugccc
ggugccggaaggucaaggggaggggugcaagccccgaaccgaagccccgg
ugaacggcggccguaacuauaacgguccuaagguagcgaaauuccuuguc
ggguaaguuccgaccugcacgaaaagcguaacgaccggagcgcugucucg
gcgagggacccggugaaauugaacuggccgugaagaugcggccuacccgu
ggcaggacgaaaagaccccguggagcuuuacugcagccugguguuggcuc
uuggucgcgccugcguaggauaggugggagccugugaacccccgccuccg
ggugggggggaggcgccggugaaauaccacccuggcgcggcugggggccu
aacccucggauggggggacagcgcuuggcgggcaguuugacuggggcggu
cgccuccuaaaagguaacggaggcgcccaaagguccccucaggcgggacg
gaaauccgccggagagcgcaaggguagaagggggccugacugcgaggccu
gcaagccgagcaggggcgaaagccgggccuagugaaccgguggucccgug
uggaagggccaucgaucaacggauaaaaguuaccccggggauaacaggcu
gaucucccccgagcguccacagcggcggggagguuuggcaccucgauguc
ggcucgucgcauccuggggcugaagaaggucccaaggguugggcuguucg
cccauuaaagcggcacgcgagcuggguucagaacgucgugagacaguucg
gucucuauccgccacgggcgcaggaggcuugaggggggcucuuccuagua
cgagaggaccggaagggacgcaccucugguuucccagcugucccuccagg
ggcauaagcuggguagccaugugcggaagggauaaccgcugaaagcaucu
aagcgggaagcccgccccaagaugaggccucccacgccgguaaggacccg
ggaagaccacccgguggaugggccggggguguaagcgccgcgaggcguug
agccgaccggucccaaucguccgaggu

..........<<<<<<<<<<<.....<<..<<<<<<<<<<<<......<<
<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....>>>
>>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<<<<
<..>>>>>>.>>....<<<<<.....<......>......>>>>>.....
......<<....>>...<<<<<.....<<.....>>....>>>>>...<<
<....<<<<<<<<.....>>>>>>>>......<<<<<<<<<....<<<..
.<<<<<.<<<<.<<<...<<<<<......>>>>>((......<<<.....
))>>>...>>>...>>>>..>>>>>..>>>>>>>>>.>>>.....<<<<<
<<<<.......>>>>>>>>>......<<<<<..((((>>>>>..>>>...
...>>.>>>>>>.>>...............<<....>>....>>......
...<<<<....>>>>..................>>>>>>>>>>>......
.<<<<<<<<<<<....>>>>>>>>>>>...<............>.<<<<<
<....<<<<<<<<<<<<<..<<<...<<<.....>>>...>>>...<<..
..>>..<<<<.....>>>>..........<......>.........>>>>
>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<
<<<......>>>>>>.....>>>...<<<..<<<<........>>>>.>>
>....>>>>>>>>>...<<<.....>>>......>>>>>>......>.>>
..<<<<<<....<<<<<....>>>>><<<<<<<<<.....<<<<<<<<<.
..<<<...<<<<<<<<....<<<<<......>>>>.>....>>>>>>>>.
.>>.>...>>>>>>>>>.....>>>>>>>.>>..<<<<<<<.<<......
>>.>>>>>>>.....<<<......>>>...<<<<.<<<...<<<(((...
..<<<<<<...........<<..<<<<<<<<<......<.<<<<<.....
.................<<......>>...>>>>>.>..>>>>>>>>>.>
>..........)))......>>>>>>.>>>...>>>>>>><<<<<<<<..
...>>>>>>>>....>>>>>><.<<<<<...<<<......<<<<<.<<<.
...>>>>>>>>...>>>..>>>>>..>.....>>>>>><......<<...
..<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<
<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...
>>>...>>...<<<<<........>>>>>))<<<<<<<<<<<<<...<<<
<....<<<<<.<<<..<<<<<.<<<<.<<..[[[[...>>>>>><<<...
<<<<<<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>>>>>>..>
>><...<<<<....>>>>.....>>>>>>...>>>..>>>>>........
.>>>>...>>...>>>>>>>>>>>>>>>..>>.....<<......>>>>>
>>.<<<<<....>>>>>..>>>>..<<<<....<<<<<<<<.<<......
...<.....<<<<<...............>>>>><<<<<<<<<<<<....
.>>>>>>>>>>>>............<<<<<<<<<..<<<.........>>
>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<<.
.<<<<<<<..<<<...<<...<<<<<....>>>>>...>>...>>>>>>>
>..>>>>><<<<<<.......>>>>>>........<...........<<<
<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>
>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<
<<<.<<<.........<<<<.<<<<<<..<<<<<.<<<.<<<<<.<<<<<
<<<<<<<<<<<...........<<<<<..<<<......<<<<<<<<....
>>>>>>>>...>>>............>>>>>..>>>>>>>>>>>>>>>>.
..<<<<<....>>>>>....>>>>>.>>>..>>>>>..<<<<.....>>>
><<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<...
....>>>>>>.....<<...(((>>....>>>>>>>...<<<<..<<<..
....>>>..>>>>.<<<....>>>.>>))).......<<<<<<<.<<..)
)))..>>>>>>>>>.............>>>>..>>>>>>.......<<<<
..<<<<<<<...<<.......>>...>>>>>>>...>>>.>.......<<
<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<<<.....>
>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>.
.......>>>>>>>>>>>....<<<<<<<...<<<<..<<<<<<<.....
..........>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<]]]]>>
>>>>...>>>>>...>>>>.>>>>>...<<<....<<<<...........
..>>>>....>>>.>>>>.......>>>>>>>..<<.>>....<<<<<<<
<<.......>>>>>....<<<<<<<<.<<<....<<<<<....>>>>>..
.>>>..>>>>>.>>>...>>>>.....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4wzo Structural insights into the translational infidelity mechanism.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): N40 G42 L43 G44 E45 T71 R72 K74 N79 F80 I88 D126 R128 N132 L133 G134 L135 R136 G154 M155 D156 A158
• Binding residue (residue number reindexed from 1): N39 G41 L42 G43 E44 T70 R71 K73 N78 F79 I87 D125 R127 N131 L132 G133 L134 R135 G153 M154 D155 A157

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4wzo, PDBe:4wzo, PDBj:4wzo
• PDBsum: 4wzo
• PubMed: 26037619
• UniProt: Q5SHQ0|RL5_THET8 Large ribosomal subunit protein uL5 (Gene Name=rplE)