Structure of PDB 4wzo Chain 41 Binding Site BS02

Receptor Information

>4wzo Chain 41 (length=181) Species: 300852 (Thermus thermophilus HB8)

PLDVALKRKYYEEVRPELIRRFGYQNVWEVPRLEKVVINQGLGEAKEDAR
ILEKAAQELALITGQKPAVTRAKKSISNFKLRKGMPIGLRVTLRRDRMWI
FLEKLLNVALPRIRDFRGLNPNSFDGRGNYNLGLREQLIFPEITYDMVDA
LRGMDIAVVTTAETDEEARALLELLGFPFRK

Ligand information

>4wzo Chain 1H (length=2902)

agaugguaagggcccacgguggaugccucggcacccgagccgaugaagga
cguggcuaccugcgauaagccagggggagccgguagcgggcguggauccc
uggauguccgaaugggggaacccggccggcgggaacgccggucaccgcgc
uuuuugcgcggggggaaccuggggaacugaaacaucucaguacccagagg
agaggaaagagaaaucgacucccugaguagcggcgagcgaaaggggacca
gccuaaaccguccggcuuguccgggcggggucguggggcccucggacacc
gaauccccagccuagccgaagcuguugggaagcagcgccagagaggguga
aagccccguaggcgaaagguggggggauaggugaggguacccgaguaccc
cgugguucguggagccaugggggaaucugggcggaccaccgccuaaggcu
aaguacuccgggugaccgauagcgcaccaguaccgugagggaaaggugaa
aagaaccccgggaggggagugaaauagagccugaaaccgugggcuuacaa
gcagucacggccccgcaagggguuguggcgugccuauugaagcaugagcc
ggcgacucacggucgugggcgagcuuaagccguugaggcggaggcguagg
gaaaccgaguccgaacagggcgcaaacggcccgcacgcgggccgaaaagu
ccgcggccguggacccgaaaccgggcgagcuagcccuggccagggugaag
cuggggugagacccaguggaggcccgaaccggugggggaugcaaaccccu
cggaugagcuggggcuaggagugaaaagcuaaccgagcccggagauagcu
gguucuccccgaaaugacuuuagggucagccucaggcgcugacuggggcc
uguagagcacugauagggcuagggggcccaccagccuaccaaacccuguc
aaacuccgaagggucccagguggagccugggagugagggcgcgagcgaua
acguccgcguccgagcgcgggaacaaccgagaccgccagcuaaggccccc
aagucugggcuaagugguaaaggauguggcgccgcgaagacagccaggau
guuggcuuagaagcagccaucauuuaaagagugcguaauagcucacuggu
cgaguggcgccgcgccgaaaaugaucggggcuuaagcccagcgccgaagc
ugcgggucugggggaugaccccaggcgguaggggagcguucccgaugccg
augaaggccgacccgcgagggcggcuggagguaagggaagugcgaaugcc
ggcaugaguaacgauaaagagggugagaaucccucucgccguaagcccaa
ggguuccuacgcaauggucgucagcguaggguuaggcgggaccuaaggug
aagccgaaaggcguagccgaagggcagccgguuaauauuccggcccuucc
cgcaggugcgauggggggacgcucuaggcuagggggaccggagccaugga
cgagcccggccagaagcgcagggugggagguaggcaaauccgccucccaa
caagcucugcgugguggggaagcccguacgggugacaaccccccgaagcc
agggagccaagaaaagccucuaagcacaaccugcgggaacccguaccgca
aaccgacacaggugggcgggugcaagagcacucaggcgcgcgggagaacc
cucgccaaggaacucugcaaguuggccccguaacuucgggagaaggggug
cucccuggggugaugagccccggggagccgcagugaacaggcucuggcga
cuguuuaccaaaaacacagcucucugcgaacucguaagaggagguauagg
gagcgacgcuugcccggugccggaaggucaaggggaggggugcaagcccc
gaaccgaagccccggugaacggcggccguaacuauaacgguccuaaggua
gcgaaauuccuugucggguaaguuccgaccugcacgaaaagcguaacgac
cggagcgcugucucggcgagggacccggugaaauugaacuggccgugaag
augcggccuacccguggcaggacgaaaagaccccguggagcuuuacugca
gccugguguuggcucuuggucgcgccugcguaggauaggugggagccugu
gaacccccgccuccgggugggggggaggcgccggugaaauaccacccugg
cgcggcugggggccuaacccucggauggggggacagcgcuuggcgggcag
uuugacuggggcggucgccuccuaaaagguaacggaggcgcccaaagguc
cccucaggcgggacggaaauccgccggagagcgcaaggguagaagggggc
cugacugcgaggccugcaagccgagcaggggcgaaagccgggccuaguga
accgguggucccguguggaagggccaucgaucaacggauaaaaguuaccc
cggggauaacaggcugaucucccccgagcguccacagcggcggggagguu
uggcaccucgaugucggcucgucgcauccuggggcugaagaaggucccaa
ggguugggcuguucgcccauuaaagcggcacgcgagcuggguucagaacg
ucgugagacaguucggucucuauccgccacgggcgcaggaggcuugaggg
gggcucuuccuaguacgagaggaccggaagggacgcaccucugguuuccc
agcugucccuccaggggcauaagcuggguagccaugugcggaagggauaa
ccgcugaaagcaucuaagcgggaagcccgccccaagaugaggccucccac
ggcgucaagccgguaaggacccgggaagaccacccgguggaugggccggg
gguguaagcgccgcgaggcguugagccgaccggucccaaucguccgaggu
cu

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<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....>>>>
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.....>>>>>>.>>...<<<<<<.....<......>......>>>>>.>.
........<<....>>...<<<<<.....<<.....>>....>>>>>...
<<<....<<<<<<<<.....>>>>>>>>......<<<<<<<<<....<<<
...<<<<<.<<<<.<<<...<<<<<......>>>>>((......<<<...
..))>>>...>>>...>>>>..>>>>>..>>>>>>>>>.>>>.....<<<
<<<<<<.......>>>>>>>>>......<<<<<..((((>>>>>..>>>.
.....>>.>>>>>>.>>...............<<....>>....>>....
.....<<<<....>>>>..................>>>>>>>>>>>....
...<<<<<<<<<<<....>>>>>>>>>>>...<............>.<<<
<<<....<<<<<<<<<<<<<..<<<...<<<.....>>>...>>>...<<
....>>..<<<<.....>>>>.....<<<<<<......>>>>>>....>>
>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<
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>>..<<<<<<....<<<<<....>>>>><<<<<<<<<.....<<<<<<<<
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..>>.>>>>>>>.....<<<......>>>...<<<<.<<<...<<<(((.
....<<<<<<...........<<..<<<<<<<<<........<<<<<.<<
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>>>>><<<<<<<<.....>>>>>>>>....>>>>>>..<<<<<...<<<.
.....<<<<<.<<<....>>>>>>>>...>>>..>>>>>........>>>
>>><......<<.....<<<<<<.......>>>>>><<<<....<<<<<.
<<...<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<.
..<<<....>>>...>>>...>>...<<<<<........>>>>>))<<<<
<<<<<<<<<...<<<<....<<<<<.<<<..<<<<<.<<<<.<<..[[[[
...>>>>>><<<...<<<<<<<<<<<<<<<<.<<.....>>>>>>>>>..
...>>>>>>>>>..>>><...<<<<....>>>>.....>>>>>>...>>>
..>>>>>.........>>>>...>>...>>>>>>>>>>>>>>>..>>...
..<<......>>>>>>>.<<<<<....>>>>>..>>>>..<<<<....<<
<<<<<<.<<.........<.....<<<<<...............>>>>><
<<<<<<<<<<<.....>>>>>>>>>>>>............<<<<<<<<<.
.<<<.........>>>..<<<<<<<.....<<<....>>>.......>>>
>>>>.<<<<<.<<<..<<<<<<<..<<<...<<...<<<<<....>>>>>
...>>...>>>>>>>>..>>>>><<<<<<.......>>>>>>........
<...........<<<<<<.....>>>>>>.....>....>>>>>..>>.>
>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<.....
...>>>>>..<<<<<<<<.<<<.........<<<<.<<<<<<..<<<<<.
<<<.<<<<<.<<<<<<.<<<<<<<<<...........<<.<<..<<<...
...<<<<<<<<....>>>>>>>>...>>>............>>.>>..>>
>>>>>>>.>>>>>>...<<<<<....>>>>>....>>>>>.>>>..>>>>
>..<<<<.....>>>><<<<<<...........>>>>>>..<<...<<<<
<<<...<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>
>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>))).....
..<<<<<<<.<<..))))..>>>>>>>>>.............>>>>..>>
>>>>.......<<<<..<<<<<<<<..<<.......>>>..>>>>>>>..
.>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>>.
.>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<
<<....>>>.>>>>........>>>>>>>>>>>....<<<<<<<...<<<
<..<<<<<<<...............>>>>>>>.<<<<<...<<<<...<<
<<<<<<<<<]]]]>>>>>>...>>>>>...>>>>.>>>>>...<<<....
<<<<<...........>>>>>....>>>.>>>>.......>>>>>>>...
................<<<<<<<<<.......>>>>>....<<<<<<<<.
<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>....>
>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4wzo Structural insights into the translational infidelity mechanism.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): K36 V38 N40 G42 L43 G44 E45 T71 A73 K74 I77 N79 F80 I88 R91 S124 D126 R128 N132 L133 G134 L135 R136 R153 G154 M155 D156 A158
• Binding residue (residue number reindexed from 1): K35 V37 N39 G41 L42 G43 E44 T70 A72 K73 I76 N78 F79 I87 R90 S123 D125 R127 N131 L132 G133 L134 R135 R152 G153 M154 D155 A157

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4wzo, PDBe:4wzo, PDBj:4wzo
• PDBsum: 4wzo
• PubMed: 26037619
• UniProt: Q5SHQ0|RL5_THET8 Large ribosomal subunit protein uL5 (Gene Name=rplE)