Structure of PDB 8cqw Chain z Binding Site BS01

Receptor Information

>8cqw Chain z (length=179) Species: 5476 (Candida albicans)

ANLRTQKRLAASVIGVGKRKVWLDPNETTEIANANSRSAIRKLYKNGTIV
KKPETVHSRSRARALKESKRAGRHMGYGKRKGTKDARMPSQVLWMRRLRV
LRKLLAKYRDAGKIDKHLYHNLYKAAKGNTFKHKRSLVEHIIAAKAEALR
EKALKEEAEARRVRNRAARERRQQRLAEK

Ligand information

>8cqw Chain 1 (length=3208)

ugaccucaaaucagguaggacuacccgcugaacuuaagcauaucaauaag
cggaggaaaagaaaccaacagggauugccucaguagcggcgagugaagcg
gcaaaagcucaaauuugaaaucuggcgucuuuggcguccgaguuguaauu
ugaagaagguaucuuugggcccggcucuugucuauguuccuuggaacagg
acgucacagagggugagaaucccgugcgaugagaugacccgggucugugu
aaaguuccuucgacgagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcuugagaucagacuugguauuuuugc
gguuuaccgggccagcaucgguuuggagcggcaggauaauggcggaggaa
uguggcacggcuucugcuguguguuauagccucugacgauacugccagcc
uagaccgaggacugcgguuaccuaggauguuggcauaaugaucuuaaguc
gcccgucuugaaacacggaccaaggagucuaacgucuaugcgaguguuug
gguguaaaacccguacgcguaaugaaagugaacgaaggugggggcccauu
agggugcaccaucgaccgauccugauguguucggauggauuugaguaaga
gcauagcuguugggacccgaaagauggugaacuaugccugaauaggguga
agccagaggaaacucugguggaggcucguagcgguucugacgugcaaauc
gaucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuagua
gcugguuccugccgaaguuucccucaggauagcagaagcucguaucaguu
uuaugagguaaagcgaaugauuagaagucuugggguugaaaugaccuuaa
cuuauucucaaacuuuaaauauguaagaaguccuuguugcuuaauugaac
guggacaauugaaugaagagcuuuuagugggccauuuuugguaagcagaa
cuggcgaugcgggaugaaccgaacgugaaguuaaagugccggaaugcacg
cucaucagacaccacaaaagguguuaguucaucuagacagccggacggug
gccauggaagucggaauccgcuaaggaguguguaacaacucaccggccga
augaacuagcccugaaaauggauggcgcucaagcgugcuacuuauacuuc
accgugauugcuguuuugacgcuuucacgaguaggcaggcguggagguca
gugacgaagccuuugcuguaaagcugggucgaacggccucuagugcagau
cuuggugguaguagcaaauauucaaaugagaacuuugaagacugaagugg
ggaaagguuccaugucaacagcaguuggacauggguuagucgauccuaag
agauggggaagcuccguuucaacgugcuugauuuuucaggccaaccaucg
aaagggaauccgguuaaaauuccggaacuuggauauggauucuucacggc
aacguaacugaauguggagacgucggcgugagcccugggaggaguuaucu
uuucuucuuaacagcuuaucacccuggaauugguuuauccggagaugggg
ucuuauggcuggaagagcgcgguaauuuugccgcguccggugcgcuuacg
acgguccuugaaaauccacaggaaggaauaguuuucaugccaagucguac
ucauaaccgcagcaggucuccaagguuaacagccucuaguugauagaaua
auguagauaagggaagucggcaaaauagauccguaacuucgggauaagga
uuggcucuaaggaucgguuuaacgaucaacuuagaacugguacggacaag
gggaaucugacugucuaauuaaaacauagcauugugauggucagaaagug
auguugacacaaugugauuucugcccagugcucugaaugucaaagugaag
aaauucaaccaagcgcggguaaacggcgggaguaacuaugacucucuuaa
gguagccaaaugccucgucaucuaauuagugacgcgcaugaauggauuaa
cgagauucccacugucccuaucuacuaucuagcgaaaccacagccaaggg
aacgggcuuggcagaaucagcggggaaagaagacccuguugagcuugacu
cuaguuugacauugugaaaagacauggaggguguagaauaagugggagcu
ucggccggugaaaccacuaccucuauaguuuuuuuacuuauucaaugaag
cggagcuggaggucaaacuccacguucuagcauuaagcccucugggcgau
ccggguugaagacauugucagguggggaguuuggcuggggcggcacaucu
guuaaacgauaacgcagguguccuaagggggacucauggagaacagaaau
cuccaguagaacaaaaggguaaaaguccccuugauuuugauuuucagugu
gaauacaaaccaugaaaguguggccuaucgauccuuuagucccucggaau
uugaggcuagaggugccagaaaaguuaccacagggauaacuggcuugugg
cagucaagcguucauagcgacauugcuuuuugauucuucgaugucggcuc
uuccuaucauaccgaagcagaauucgguaagcguuggauuguucacccac
uaauagggaacgugagcuggguuuagaccgucgugagacagguuaguuuu
acccuacugaugaauguuaucgcaauaguaauugaacuuaguacgagagg
aaccguucauucagauaauugguuuuugcggcugucugaucaggcaacgc
cgcgaagcuaccaucugcuggauuauggcugaacgccucuaagucagaau
ccaugcuagaacgcgaugauuuuugcccugcacauuuuagauggauacga
auaagacuuuuuagucgcuggaccauagcaggcuggcaacggugcgcuua
gcggaaaggcuuugugcgcuugccggcggauagcaaugucaacaugcgcg
gggauaaauccuuugcauacgacuuagauguacaacggaguauuguaagc
aguagaguagccuuguuguuacgaucugcugagauuaagcucuuguuguc
ugauuugu

.......................<<<<<<.....<......>......>>
>>>.>.........<<....>>..<<<<<......<<.....>>.....>
>>>>..[[[...........<<.<<<<<<...>>>>>>.>>.........
....<<<<<...<<<<<<<<<<<<.<<<<<<<<....<<<<......>>>
>.(.......<<<......)>>>..>.>>>>>>>....>>>>>>>>....
>>>>..>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
.<.<<....>>...>...........<<<<....>>>>............
......................<<<<<<<<<<<<.....<<<<..<....
.>..>>>>..<<<<<<<.<<<<<<<<.<<<<<<<....<<.<<<<<<<..
....<<<<<<......>>>>>>.......>>>>>>.>.>>.>>>>>.>>>
>>>>>>>........<<<.>>>.....>>>>>>>....>>>>>>>>>>>>
..<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<..<<
<<<.....>>>>>..>>>...<<....>>.......<<<<<..<<<<...
.>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>....
>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<<....>>>>>>>.....>>>...<<<<.<<.<<........>>
>>>>>>....>>>>>>>>>...<<.......>>......>>>>>>...>.
.>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<...<<<.<<
<<<<<.....<<<....<<<....<<<<...<<<<<<.....>>>>>>.>
>>>....>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>
>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<.....>>>>>
.>>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<<
<.....<<..........<<<.<<<<<<<<<<.......<<<<<.<<...
.<<.........>>....>>.........<<......>>...>>>>>...
>>>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>>>>>
>><<<<<..<<.........>>..>>>>>....>>>>>>..<<<<<<<<.
<<......<<<<..<<<....>>>.>>>>...>>>>>>>>>>........
>>>>>><<.....<<.....<<<<<<.......>>>>>><<<<.....<<
<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<
<....<<<....>>>..........<<<<<<....>>>>>>......>>.
...>>...<<<<<........>>>>>))<<<<.<<<<<<<.....<<<..
..>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..{{{.
.>>>>>><...<<<<<....<<<<<<....<<<.....>>>....>>>>>
>.....>>>>>....><<<<<<<......>>>>>>>>>>>...>>>>>>>
.>>>>.........>>>>>>..........>>>..>>>>>>>>>>>>..>
>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<...
..<<<<<<<.<<...............<<<<<....<....>.....>>>
>><<.<<<<<<<<<<<......>>>>>..>>>>>>.>>............
<<<<<<<<<..<<<.........>>>..<<<<<<<....<<<<.....>>
>>......>>>>>>>...<<..<<<..<<<<<<....<<<.....<<<<.
...>>>>.....>>>.>>>>..>>>>><<<<<<.......>>>>>>....
....<...........<<<<<.......>>>>>.....>.....>>....
>>.>>>>>>>.....>>.>>>>>>>..>>>>>>....>>....<<<<<..
......>>>>>....[[[[[..{{{.{{......[[[[.{{[[[[..<<<
<<.<<<<<<<<..<<<<<<<<<<...<.<<....................
....................>>.>...>>>>>>>>>>...<<<<<.....
<<<<..<<<<<......>>>>>..............<<<<...>>>>..>
>>>..>>>>>.....>>>>>>>>..>>>>>...<<<.....>>>..<<<<
<<...........>>>>>>..<<...<<<<<<<...<<<<<<.......>
>>>>>.....<<...(((>>....>>>>>>>...<<<.<<<<<.......
>>>>.>>>>.<<<....>>>.>>))).......((((((..((((.>>>>
>>))))))))))..........]]]]..}}]]]].......<<.<...<<
<<<<...<<.......>>...>>>>>>....>.>.>.......<<<<<.<
<<<<<..<.<<<<<<<......>>>>>>>...>.<<<.........>>>.
....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....}}
..}}}.]]]]]....<<<<<<<<..<<<<...<<<<<.............
....>>>>>..<<<<<...<<<<....<<<<<<<<<<}}}.>>>>>..>>
>>>...>>>>..>>>>>.<<<<...<<<<<...........>>>>>...>
>>>.>>>>....>>>>>>>>.....<<<<<<.<<<.<.....<<......
....<<<......>>>......>>.......<<<<<<...<<<<<<<..<
<<......>>>..>>>>>>>.>>>>>>..............>.>>>.>>>
>>>..<<<<<<<.............>>....<<<<<<<<<.<<<....<<
<<.<..<<<<<......>>>>>..>>>>>...>>>....>>>>.>>>>>.
.>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8cqw Drug-induced rotational movement of the ribosome is a key factor for read-through enhancement
• Resolution: 3.05 Å
• Binding residue (original residue number in PDB): A2 N3 L4 R5 T6 R9 G18 K19 R20 K21 W23 L24 P26 S37 R38 S39 R42 K43 K46 E55 T56 V57 H58 S59 R60 S61 R62 A63 R64 K70 R74 H75 G77 Y78 G79 K80 R81 K82 G83 T84 K85 R88 M89 P90 Q92 W95 M96 R97 L99 R100 V101 R103 K104 R110 G113 K114 K117 H118 Y120 H121 Y124 K125 K128 G129 N130 K135 R136
• Binding residue (residue number reindexed from 1): A1 N2 L3 R4 T5 R8 G17 K18 R19 K20 W22 L23 P25 S36 R37 S38 R41 K42 K45 E54 T55 V56 H57 S58 R59 S60 R61 A62 R63 K69 R73 H74 G76 Y77 G78 K79 R80 K81 G82 T83 K84 R87 M88 P89 Q91 W94 M95 R96 L98 R99 V100 R102 K103 R109 G112 K113 K116 H117 Y119 H120 Y123 K124 K127 G128 N129 K134 R135

External links

• PDB: RCSB:8cqw, PDBe:8cqw, PDBj:8cqw
• PDBsum: 8cqw
• UniProt: A0A1D8PK40|RL19A_CANAL Large ribosomal subunit protein eL19 (Gene Name=RPL19A)