Structure of PDB 7ohr Chain y Binding Site BS01

Receptor Information

>7ohr Chain y (length=225) Species: 559292 (Saccharomyces cerevisiae S288C)

MATRTQFENSNEIGVFSKLTNTYCLVAVGGSENFYSAFEAELGDAIPIVH
TTIAGTRIIGRMTAGNRRGLLVPTQTTDQELQHLRNSLPDSVKIQRVEER
LSALGNVICCNDYVALVHPDIDRETEELISDVLGVEVFRQTISGNILVGS
YCSLSNQGGLVHPQTSVQDQEELSSLLQVPLVAGTVNRGSSVVGAGMVVN
DYLAVTGLDTTAPELSVIESIFRLQ

Ligand information

>7ohr Chain 1 (length=2373)

guuugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaau
aagcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaa
gcggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguugua
auuuggagagggcaacuuuggggccguuccuugucuauguuccuuggaac
aggacgucauagagggugagaaucccguguggcgaggagugcgguucuuu
guaaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugg
gugguuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaagu
acagugauggaaagaugaaaagaacuuugaaaagagagugaaaaaguacg
ugaaauuguugaaagggaagggcauuugaucagacaggccagcaucaguu
uugguggcaggauaaauccauaggaauguagcuugccucgguaaguauua
uagccugugggaauacugccagcugggacugaggacugcgacguaaguca
aggaugcuggcauaaugguuauaugccgcccgucuugaaacacggaccaa
ggagucuaacgucuaugcgaguguuuggguguaaaacccauacgcguaau
gaacgagguuggggccucgcaagaggugcacaaucgaccgauccugagga
uggauuugguaagagcauagcuguugggacccgaaagauggugaacuaug
ccugaauagggugaagccagaggaaacucugguggagcucgguucgacgu
ucgaucgugaauuggguauagggcgaaagacaaccaucuaguagcugguu
ccugccgaaguuucccuaggauagcagaagcucguaucaguuuuaugagu
aagaaugaagagcuuuuagugggccauuuuugguaagcagaacuggcgau
gcgggaugaaccgaacguagaguuaaggugccggaauacacgcucaucag
acacaaaagguguuaguucaucuagacagccggacgguggccauggaagu
cggaauccgcuaaggaguguguaacaacucaccggccgaaugaacuagcc
cugaaaauggauggcgcucaagcguguuaccuauacucuaccgucagggu
ugauaugaugcccugacgaguaggcaggcguggaggucagugacgaagcc
uagaccguaaggucgggucgaacggccucuagugcagaucuuggugguag
uagcaaauauucaaaugagaacuuugaagacugaaguggggaaagguucc
acgucaacagcaguuggacguggguuagucgauccuaagagauggggaag
cuccguuucaaaggccugauuuuaugcaggccaccaucgaaagggaaucc
gguuaagauuccggaaccuggauauggauucuucacgguaacguaacuga
auguggagacgucggcgcgagcccugggaggaguuaucuuuucuucuuaa
cagcuuaucaccccggaauugguuuauccggagauggggucuuauggcug
gaagaggccagcacugcuggcuccggugcgcuugugacggccgugaaaau
ccacaggaaggaauaguuuucaugccaggucguacugggucuccaaggug
aacagccucuaguugauagaauaauguagauaagggaagucggcuguccc
uaucuacuaucuagcgaaaccacagccaagggaacgggcuuggcagaauc
agcggggacccuguugagcuugacucuaguuugacauuauugucaggugg
ggaguaaaguuaccacagggauaauggcuuguggcagucaagcguucagc
gacauugcucggcucuuccuaucauaccgaagcagaauucgguaagcguu
ggauuguucacccacuaauagggaacguggcuggguagaccgucgugaga
cgguuaguuuuacccuacugaugaauguuaccgcaauaguaauugaacuu
aguacgagaggaacaguucauucggauaauugguuuuugcggcugucuga
ucaggcaugccgcgaagcuaccauccgcuggauuauggcugaacgccucu
aagucagaauccaugcuagaacgcggugauuucuuugcuccacacaauau
agauggauacgaauaaggcguccuuguggcgucgcugaaccauagcaggc
uagcaacggugcacuuggcggaaaggccuugggugcuugcuggcgaauug
caaugucauuuugcguggggauaaaucauuuguauacgacuuagauguac
aacgggguauuguaagcaguagaguagccuugguuacgaucugcugagau
uaagccuuuguugucugauuugu

..........................<<<<<<.....<......>.....
.>>>>>.>...<.....<<....>>..<<<<<......<<.....>>...
..>>>>>..<<<..<........<<.<<<<<<...>>>>>>.>>......
..>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......
>>>>.((......<<<.....))>>>....>>>>>>>>>..>>>>>>>..
..>>>>>>>>>>>........<<<<<<<........>>>>>>>.....<<
<<<<..>>>>>>..>>>....>............................
.<<....>>...............<<<<....>>>>..............
....................<<<<<.<<<<<......<<<<<<<.<<<<<
<<<<<<<<<<.....<<<<<<<<<......<<<<<<....>>>>>>....
...>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>>>.
....>>>>>>>.....>>>>>.>>>>>..<<<<........>>>><<<<<
<....<<<<<<.<<<<<<..<<<...<<<<.....>>>>...>>>.....
......<<<<<..<<<<<....>>>>>...>>>>>.<<<<<<<<......
.>>>.>>>>>....>>>>>>.>>>>>>..<<.<..<<<<<<<...<<<<<
<<<<....<<<<...<<<<<<<....>>>>>>>...>>>>...<<<....
.>>>........>>>>>>>>>...........>>>>>>>.....>.>>..
<<<<<<....<...........><<<<<<<<<....<<<.<<<<<<..>>
>>.>>>>>..>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>...
.<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<.....<<
.......<<<.<<<<<<<<<<.......<<<<<.<<<...<<........
.>>...>>>.........<<......>>...>>>>>...>>>>>>>>>>>
>>..........))).>>...>>>>>>.>>>>..>>>>>>><<<<<<<<<
.........>>>>>>>>>....>>>>>>..<<<<<<<..<<......<<<
<.<<<<....>>>>>>>>...>>.>>>>>>>........>>>>>><<...
..<<.....<<<<<<.......>>>>>><<<<.....<<<<<.<<...<<
<<<<...<<.....>>.>>>>>>....((<<<<<<...<<....<<<...
.>>>........<<<<<<........>>>>>>....>>....>>...<<<
<<........>>>>>))<<<<.<<<<<<<.....<<<....>>>...<..
.<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..(((..>>>>>><...
<<<<<....<<<<<<....<<<.....>>>....>>>>>>.....>>>>>
....><<<<<<<...>>>>>>>>>>>...>>>>>>>.>>>>........>
>>>>>...>......>.>>.>>>>>>>>>>>>..>>...>>>>>.<<<<.
....>>>>..>>>>..<<<<.....<<<<<<<.<<............>>.
>>>>>>>..>>>>>>....>>....<<<<<........>>>>>....<<<
<<..<<..<<<<<.<..<<<<..<<<<.<<<<<<<<....>>>>>>>>..
>>>>..>>>>....>>>>>>............<<<<<....<<.....>>
....>>>>>.<<...<<<<<..<.<<<<<<<......>>>>>>>...>.<
<<.........>>>.....>>>>>......>>.....<<<<<<<....>>
>>>>>.........>>.>>>>>....<<<<<<<<..<<<<.<.<<<<<<.
..............>>>>>>.><<<<<...<<<<...<<<<<<.<<<<))
).>>>>..>>>>>>..>>>>..>>>>>.<<<<...<<<<<..........
.>>>>>...>>>>.>>>>....>>>>>>>>.......<<<<<<.<<<.<<
....<<..........<<<<<<.....>>>>>>......>>.......<<
<<<<...<<<<<.<..<<<......>>>..>.>>>>>.>>>>>>......
.......>>.>>>.>>>>>>..<<<<<.<.............>.....<<
<<<.<<<.<<<....<<<<....<..<<<..>>>..>...>>>>...>>>
....>>>..>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohr Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 4.72 Å
• Binding residue (original residue number in PDB): E8 N9 S31 N33
• Binding residue (residue number reindexed from 1): E8 N9 S31 N33

Gene Ontology

Molecular Function

• GO:0003743 translation initiation factor activity
• GO:0005515 protein binding
• GO:0043022 ribosome binding
• GO:0043023 ribosomal large subunit binding

Biological Process

• GO:0000054 ribosomal subunit export from nucleus
• GO:0000460 maturation of 5.8S rRNA
• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000466 maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000470 maturation of LSU-rRNA
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0006413 translational initiation
• GO:0042254 ribosome biogenesis
• GO:0042256 cytosolic ribosome assembly
• GO:0042273 ribosomal large subunit biogenesis
• GO:1902626 assembly of large subunit precursor of preribosome

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:7ohr, PDBe:7ohr, PDBj:7ohr
• PDBsum: 7ohr
• PubMed: 34813592
• UniProt: Q12522|IF6_YEAST Eukaryotic translation initiation factor 6 (Gene Name=TIF6)