Structure of PDB 8bqd Chain x Binding Site BS01

Receptor Information

>8bqd Chain x (length=367) Species: 4932 (Saccharomyces cerevisiae)

YCSGLQCGRETSSQMKCPVCLKQGIVSIFCDTSCYENNYKAHKALHNAKD
GLEGAYDPFPKFKYSGKVKASYPLTPRRYVPEDIPKPDWAANGLPVSEQR
NDRLNNIPIYKKDQIKKIRKACMLGREVLDIAAAHVRPGITTDELDEIVH
NETIKRGAYPSPLNYYNFPKSLCTSVNEVICHGVPDKTVLKEGDIVNLDV
SLYYQGYHADLNETYYVGENISKEALNTTETSRECLKLAIKMCKPGTTFQ
ELGDHIEKHATENKCSVVRTYCGHGVGEFFHCSPNIPHYAKNRTPGVMKP
GMVFTIEPMINEGTWKDMTWPDDWTSTTQDGKLSAQFEHTLLVTEHGVEI
LTARNKKSPGGPRQRIK

Ligand information

>8bqd Chain BQ (length=3299)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
uuucacuggccagcaucaguuuugguggcaggauaauccauaggaaugua
gcuugccucgguaaguauuauagccugugggaauacugccagcugggacu
gaggacugcgacguaagucaaggaugcuggcauaaugguuauaugccgcc
cgucuugaaacacggaccaaggagucuaacgucuaugcgaguguuugggu
guaaaacccauacgcguaaugaaagugaacguagguuggggccucgcaag
aggugcacaaucgaccgauccugaugucuucggauggauuugaguaagag
cauagcuguugggacccgaaagauggugaacuaugccugaauagggugaa
gccagaggaaacucugguggaggcucguagcgguucugacgugcaaaucg
aucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuaguag
ugguuccugccgaaguuucccucaggauagcagaagcucguaucaguuuu
augagguaaagcgaaugauuagagguuccggggucgaaaugaccuugacc
uauucucaaacuuuaaauauguaagaaguccuuguuacuuaauugaacgu
ggacauuugaaugaagagcuuuuagugggccauuuuugguaagcagaacu
ggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacacgcu
caucagacaccacaaaagguguuaguucaucuagacagccggacgguggc
cauggaagucggaauccgcuaaggaguguguaacaacucaccggccgaau
gaacuagcccugaaaauggauggcgcucaagcguguuaccuauacucuac
cgucaggguugauaugaugcccugacgaguaggcaggcguggaggucagu
gacgaagccuagaccguaaggucgggucgaacggccucuagugcagaucu
uggugguaguagcaaauauucaaaugagaacuuugaagacugaagugggg
aaagguuccacgucaacagcaguuggacguggguuagucgauccuaagag
auggggaagcuccguuucaaaggccugauuuuaugcaggccaccaucgaa
agggaauccgguuaagauuccggaaccuggauauggauucuucacgguaa
cguaacugaauguggagacgucggcgcgagcccugggaggaguuaucuuu
ucuucuuaacagcuuaucaccccggaauugguuuauccggagaugggguc
uuauggcuggaagaggccagcaccuuugcuggcuccggugcgcuugugac
ggcccgugaaaauccacaggaaggaauaguuuucaugccaggucguacug
auaaccgcagcaggucuccaaggugaacagccucuaguugauagaauaau
guagauaagggaagucggcaaaauagauccguaacuucgggauaaggauu
ggcucuaagggucggguagugagagccuuggucagacgcagcgggcgugc
uuguggacugcuugguggggcuugcucugcuaggcggacuacuugcgugc
cuuguuguagacggccuuggucuugcuacaauuaacgaucaacuuagaac
ugguacggacaaggggaaucugacugucuaauuaaaacauagcauugcga
uggucagaaagugauguugacgcaaugugauuucugcccagugcucugaa
ugucaaagugaagaaauucaaccaagcgcggguaaacggcgggaguaacu
augacucucuuaagguagccaaaugccucgucaucuaauuagugacgcgc
augaauggauuaacgagauucccacugucccuaucuacuaucuagcgaaa
ccacagccaagggaacgggcuuggcagaaucagcggggaaagaagacccu
guugagcuugacucuaguuugacauugugaagagacauagagggcuaccu
uuauaguuucuuuacuuauucaaugaagcggagcuggaauucauuuucca
cguucuagcauucaaggucccauucggggcugauccggguugaagacauu
gucagguggggaguuuggcuggggcggcacaucuguuaaacgauaacgca
gauguccuaaggggggcucauggagaacagaaaucuccaguagaacaaaa
ggguaaaagcccccuugauuuugauuuucagugugaauacaaaccaugaa
aguguggccuaucgauccuuuagucccucggaauuugaggcuagaggugc
cagaaaaguuaccacagggauaacuggcuuguggcagucaagcguucaua
gcgacauugcuuuuugauucuucgaugucggcucuuccuaucauaccgaa
gcagaauucgguaagcguuggauuguucacccacuaauagggaacgugag
cuggguuuagaccgucgugagacagguuaguuuuacccuacugaugaaug
uuaccgcaauaguaauugaacuuaguacgagaggaacaguucauucggau
aauugguuuuugcggcugucugaucaggcauugccgcgaagcuaccaucc
gcuggauuauggcugaacgccucuaagucagaauccaugcuagaacgcgg
ugauuucuuugcuccacacaauauagauggauacgaauaaggcguccuug
uggcgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaagg
ccuugggugcuugcuggcgaauugcaaugucauuuugcguggggauaaau
cauuuguauacgacuuagauguacaacgggguauuguaagcaguagagua
gccuuguuguuacgaucugcugagauuaagccuuuguugucugauuugu

........................<<<<<<.....<......>......>
>>>>.>.........<<....>>..<<<<<......<<.....>>.....
>>>>>..[[[..<<.......<<.<<<<<<...>>>>>>.>>.......>
>....<<<<<<<.<<<<<<<<<<<<<<<<<<<<.....<<<<......>>
>>.(.......<<<......)>>>....>>>>>>>>>..>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(..<<<<<<)))))..]]]...............................
...<<....>>...............<<<<....>>>>............
......................<<<<<.<<<<<.................
........<<<<<<<.<<<<<<<<<<<<<<<.....<<<<<<<<......
<<<<<<....>>>>>>.......>>>>>>>.>...>>>>>.>>>>>>>>>
>........<<<....>>>.....>>>>>>>.....>>>>>.>>>>>..<
<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<...<<<<
.....>>>>...>>>...<<....>>.......<<<<<..<<<<<....>
>>>>...>>>>>.<<<<<<<<...<<....>>...>>>.>>>.>>....>
>>>>>.>>>>>>..<<....<<<<<<<.....<<<<<<<<<..<<<....
<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<........>>>
>.>>>....>>>>>>>>>...<<.......>>......>>>>>>...>..
.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<....<<.<<<<
<<<.....<<<....<<<..<.<<<<...<<<<<<.....>>>>>>.>>>
>.>..>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>>.
>>>>.....>>>>..>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>
>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<<<.
....<<..........<<<.<<<<<<<<<<.......<<<<<.<<<...<
<.........>>...>>>.........<<......>>...>>>>>...>>
>>>>>>>>>>>..........))).>>...>>>>>>.>>>>..>>>>>>>
<<<<<<<<<.........>>>>>>>>>....>>>>>><<<<<<<<<<.<<
......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>.>>.....>>
>>>><<.....<<.....<<<<<<.......>>>>>><<<<.....<<<<
<.<<...<<<<<<...<<.....>>.>>>>>>....((<<<<<<...<<.
...<<<....>>>........<<<<<<........>>>>>>....>>...
.>>...<<<<<........>>>>>))<<<<.<<<<<<<.....<<<....
>>>.......<<<<<<...<<<<.<<<<<<<.<<<<<<<<<<..{{{..>
>>>>><...<<<<<....<<<<<<....<<<.....>>>....>>>>>>.
....>>>>>....><<<<<<<<....>>>>>>>>>>>>...>>>>>>>.>
>>>.........>>>>>>..........>>>..>>>>>>>>>>>>..>>.
....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<.....
<<<<<<<.<<.........<.....<<<<<...............>>>>>
<<.<<<<<<<<<<<..<<<<<......<..<<......<...<<<<.<.<
..<<<..<<<<..<<<<<<<....>>>>>>>..>>>>..>>>..>.>.>>
>.>...>......>>...>....>>>>>.......>>>>>..>>>>>>.>
>............<<<<<<<<<..<<<.........>>>..<<<<<<<..
..<<<<.....>>>>......>>>>>>>...<<..<<<..<<<<<<....
.<<....<<<<<....>>>>.>...>>..>>>>..>>>>><<<<<<....
...>>>>>>........<...........<<<<<.......>>>>>....
.>.....>>....>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....
>>....<<<<<........>>>>>....[[[[[..{{{.{{......[[[
[.{{[[[[..<<<<<.<<<<<<<<..<<<<<<<<<<<<<<<<......>>
>>>>.>>>>>>>>>>...<<<<<.....<<<<..<<<<........>>>>
...............<<<<<<....>>>>>>..>>>>..>>>>>.....>
>>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........>>>
>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<...(
((>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<...
.>>>.>>))).......((((((..((((.>>>>>>))))))))))....
......]]]]..}}]]]].......<<.<.<.<<<<<<...<<.......
>>...>>>>>>...>>.>.>.......<<<<<.<<<<<<..<.<<<<<<<
......>>>>>>>...>.<<<.........>>>.....>>>>>..>...>
>>.>>....<<<<<<<....>>>.>>>>....}}..}}}.]]]]]....<
<<<<<<<..<<<<.<.<<<<<<...............>>>>>>.><<<<<
...<<<<....<<<<<<<<<<}}}.>>>>>..>>>>>...>>>>..>>>>
>.<<<<...<<<<<...........>>>>>...>>>>.>>>>....>>>>
>>>>.......<<<<<<.<<<.<<....<<..........<<<<<<....
.>>>>>>......>>.......<<<<<<...<<<<<.<..<<<......>
>>..>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<<<<
<.<.............>.....<<<<<<<<<.<<<....<<<<.<..<<<
<........>>>>..>>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8bqd The dynamic architecture of Map1- and NatB-ribosome complexes coordinates the sequential modifications of nascent polypeptide chains.
• Resolution: 3.9 Å
• Binding residue (original residue number in PDB): A68 K69 T281 K352
• Binding residue (residue number reindexed from 1): A48 K49 T261 K332

Enzymatic activity

• Enzyme Commision number: 3.4.11.18: methionyl aminopeptidase.

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0004177 aminopeptidase activity
• GO:0004239 initiator methionyl aminopeptidase activity
• GO:0008270 zinc ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0010629 negative regulation of gene expression
• GO:0016485 protein processing

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0010494 cytoplasmic stress granule
• GO:0022626 cytosolic ribosome

External links

• PDB: RCSB:8bqd, PDBe:8bqd, PDBj:8bqd
• PDBsum: 8bqd
• PubMed: 37079644
• UniProt: Q01662|MAP1_YEAST Methionine aminopeptidase 1 (Gene Name=MAP1)