Structure of PDB 7unv Chain x Binding Site BS01

Receptor Information

>7unv Chain x (length=593) Species: 208964 (Pseudomonas aeruginosa PAO1)

HGFQNPTGPIVREVNIGETITVAELAAQMSVKGAEVVKFMFKMGSPVTIN
QVLDQETAQLVAEELGHKVKLVSENALEEQLAESLKFEGEAVTRAPVVTV
MGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVETERGMVTFLDTP
GHAAFTAMRARGAQATDIVILVVAADDGVMPQTQEAVQHAKAAGVPIVVA
VNKIDKPEANPDNIKNGLAALDVIPEEWGGDAPFVPVSAKLGTGVDELLE
AVLLQAEVLELKATPSAPGRGVVVESRLDKGRGPVATVLVQDGTLRQGDM
VLVGINYGRVRAMLDENGKPIKEAGPSIPVEILGLDGTPDAGDEMTVVAD
EKKAREVALFRQGKFREVKLARAHAGKLENIFENMGQEEKKTLNIVLKAD
VRGSLEALQGSLSGLGNDEVQVRVVGGGVGGITESDANLALASNAVLFGF
NVRADAGARKIVEAEGLDMRYYNVIYDIIEDVKKALTGMLGSDLRENILG
IAEVRDVFRSPKFGAIAGCMVTEGMVHRNRPIRVLRDDVVIFEGELESLR
RFKDDVAEVRAGMECGIGVKSYNDVKVGDKIEVFEKVEVARSL

Ligand information

>7unv Chain a (length=1522)

gaacugaagaguuugaucauggcucagauugaacgcuggcggcaggccua
acacaugcaagucgagcggaugaagggagcuugcuccuggauucagcggc
ggacgggugaguaaugccuaggaaucugccugguagugggggauaacguc
cggaaacgggcgcuaauaccgcauacguccugagggagaaagugggggau
cuucggaccucacgcuaucagaugagccuaggucggauuagcuaguuggu
gggguaaaggccuaccaaggcgacgauccguaacuggucugagaggauga
ucagucacacuggaacugagacacgguccagacuccuacgggaggcagca
guggggaauauuggacaaugggcgaaagccugauccagccaugccgcgug
ugugaagaaggucuucggauuguaaagcacuuuaaguugggaggaagggc
aguaaguuaauaccuugcuguuuugacguuaccaacagaauaagcaccgg
cuaacuucgugccagcagccgcgguaauacgaagggugcaagcguuaauc
ggaauuacugggcguaaagcgcgcguaggugguucagcaaguuggaugug
aaauccccgggcucaaccugggaacugcauccaaaacuacugagcuagag
uacgguagagggugguggaauuuccuguguagcggugaaaugcguagaua
uaggaaggaacaccaguggcgaaggcgaccaccuggacugauacugacac
ugaggugcgaaagcguggggagcaaacaggauuagauacccugguagucc
acgccguaaacgaugucgacuagccguugggaucgaucuuaguggcgcag
cuaacgcgauaagucgaccgccuggggaguacggccgcaagguuaaaacu
caaaugaauugacgggggcccgcacaagcgguggagcaugugguuuaauu
cgaagcaacgcgaagaaccuuaccuggccuugacaugcugagaacuuucc
agagauggauuggugccuucgggaacucagacacaggugcugcauggcug
ucgucagcucgugucgugagauguuggguuaagucccguaacgagcgcaa
cccuuguccuuaguuaccagcaccucgggugggcacucuaaggagacugc
cggugacaaaccggaggaagguggggaugacgucaagucaucauggcccu
uacggccagggcuacacacgugcuacaauggucgguacaaaggguugcca
agccgcgagguggagcuaaucccauaaaaccgaucguaguccggaucgca
gucugcaacucgacugcgugaagucggaaucgcuaguaaucgugaaucag
aaugucacggugaauacguucccgggccuuguacacaccgcccgucacac
caugggaguggguugcuccagaaguagcuagucuaaccgcaagggggacg
guuaccacggagugauucaugacuggggugaagucguaacaagguagccg
uaggggaaccugcggcuggauc

........<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<....
.<<<.<<<..<<<..<<.<<....<<<<<<..>>>>>>....>>.>>>>>
......<<.......<<<<<<<..<<...<<<<<<<<<.<<.....<<<<
<<....>>>>>>......>>.>.....<<<....>>>....<<<<<<..<
<....>>>>>>>>>>>>>>>>..>>.>>>>>>><<<....<<<..<<<<<
<<<.......>>>>>>>>>>>......>>>..<<<<<<<<....>>>>..
.>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>...>>>
>>>.........<<<....<<<<....>>>>..>>>..>>.>>>>>>..<
<<<......<<<<....>>>>.....>>>>...<.<<<<<......<<<<
<<<<<<.......>>>>>>>>>>........>>>>>....>..<<<<<((
(...<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>>>>>>
..........<<<((.....<<<<...<<<.<<<<<<..<<<<<<<<<<.
.....<<<<<<.....>>>>>>....>>>>>>>>..>>.>>>>>>...<<
<<<<<<...<<<<<<<....<<<<<<<....<<<......>>>.......
>>>>>>.>..........<<....>>.>>>>>>>..>>>>.>>>>...>>
>...>>>>....<<<<<<...<<...<<<<.<.....>.>>>>...>>>>
>>>>..........<<<<<<..<<<<<<<<<<<..>>>>>>>>>>>...<
<..))>>.....>>>>>>.>>>.<<<......<<<<....>>>>....>>
>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<<<<......
<<........>>..........<<<<<<<......<<<<<<<..<...<<
<....>>>...>...<<....>>..>>>>>.>>.<<<.<<<..<<<<<<.
......<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>....
.<<<<.<<<<<<<...<<..<<<.....>>>>>....>>>>>>>.....<
<<<<.....>>>>>........>>>>.........>>>...>>>>>>>>>
...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<..<<..
.<<<....>>>...>>....>>>.....>>>>>>>......<....<<<<
<<<........>>>>>>>....>.....>>>>>>....<<<<<<<.....
....>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.<<<
<.<<<..<<<<<<<<<<<<....<<<<<<.<<<<..<<....>>.>>>>>
>>>>>...>>>>>>>>>>>>..>>>.>>>>..>.>>...>.....<<<<<
<<<<....>>>>>>>>>.....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7unv Compact IF2 allows initiator tRNA accommodation into the P site and gates the ribosome to elongation
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): F250 R259 S277 K279 K527 G528 G530 R556 R558 R602 E681 S682
• Binding residue (residue number reindexed from 1): F3 R12 S30 K32 K280 G281 G283 R309 R311 R355 E434 S435

Gene Ontology

Molecular Function

• GO:0003743 translation initiation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding

Biological Process

• GO:0006412 translation
• GO:0006413 translational initiation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7unv, PDBe:7unv, PDBj:7unv
• PDBsum: 7unv
• UniProt: Q9HV55|IF2_PSEAE Translation initiation factor IF-2 (Gene Name=infB)