Structure of PDB 7oht Chain x Binding Site BS01

Receptor Information

>7oht Chain x (length=488) Species: 559292 (Saccharomyces cerevisiae S288C)

STLIPPPSKKQKKEAQLPREVAIIPKDLPNVSIKFQALDTGDNALRVPGA
ISEKQLEELLNQLNGTSVPYTFSCTIKASDPYSSLIKPQITLLYTPRAVF
KVKPVTRSSSAIAGHGSTILCSAFAPHTSSRMVTGAGDNTARIWDCDTQT
PMHTLKGHYNWVLCVSWSPDGEVIATGSMDNTIRLWDPKSGQCLGDALRG
HSKWITSLSWEPIHLVKPGSKPRLASSSKDGTIKIWDTVSRVCQYTMSGH
TNSVSCVKWGGQGLLYSGSHDRTVRVWDINSQGRCINILKSHAHWVNHLS
LSTDYALRIGAFDHTGKKPSTPEEAQKKALENYEKICKKNGNSEEMMVTA
SDDYTMFLWNPLKSTKPIARMTGHQKLVNHVAFSPDGRYIVSASFDNSIK
LWDGRDGKFISTFRGHVASVYQVAWSSDCRLLVSCSKDTTLKVWDVRTRK
LSVDLPGHKDEVYTVDWSVDGKRVCSGGKDKMVRLWTH

Ligand information

>7oht Chain 1 (length=1485)

cuauuggaacaggacgucauagagggugagaaucccgugugggaccgaua
guaagaacugagagugaaaaaguacgugaaauuguugacaggccagcauc
aguuuugguggcaggauaaauccauaggaauguagcuugccugguaagua
uuauagccugugggaauacugccagcugggacugaggacugcgacguaag
ucaaggaugcuggcauugcgaguguuuggguguaaacccauacgcgaacg
uaggguccugggauaagagcauaguuccugccgaaguuucccucaggaua
gcagaagcucguaucaguuuuaugagguaaagcgaacuuuaaauauguaa
gaaguccuuguuugaacguggacauuugaaugaagagcuuuuagugggcc
auuuuugguaagcagaacuggcgaugcgggaugaaccgaacguagaguua
aggugccggaauacacgcucaucagacacacaaaagguguuaguucaucu
agacagccggacgguggccauggaagucggaauccgcuaaggagugugua
acaacucaccggccgaaugaacuagcccugaaaaggauggcgcucaagcg
uguuaccuauacucuaccgucaggguuaugcccugacgaguaggcaggcg
uggaggucagugagaagccugacaaggucgggucgaacggccucuaguga
aucagcgggggaagacccuguugagcuugacucuagguggggaguuuggg
ggcggcacaucuguuaaacgauaagcagauguccuaaggggggcucaugg
agaacagaaaucuccaguagaacaaaaggaaaagcccccuugauuuugau
uuucagugugaauacaaaccaugaaaguguggccuaucgauccuuuauag
aggugccagaaaaguuaccacagggauaacuggcuuguggcagucaagcg
uuagcgacauugcuuuugauucuucgaugucggcucuuccuaucauaccg
aagcagaauucgguaagcguuggauuguucacccacuaauagggagucug
gguuuagaccgucgugagacagguuaguuccuacugaugaauguuaccgc
aauaguaauugaacuuaguacgagaggaacaguucauucggauaauuggu
uuuugcggcugucugaucaggcaugccgcgaagcuaccauccgcuggauu
auggcugaacgccucuaagucagaauccaugcuagaacgcggugauuucu
ugcuccacacaauauagauggauacgaauaaggcguccuuguggcgucgc
ugaaccauagcaggcuagcaacggugcacuuggcggaaaggccuugggug
cuugcuggcgaauugcaaugucauuuugcguggggauaaaucauuuguau
acgacuuagauguacaacgggguauuguaagcaguagaguagccguuacg
aucugcugagauuaagccuuuguugucugauuugu

<..............((......<<<.....))>>>....>.........
.......<...>.............................<<<<<<<.<
<<<<<<<<<<<<<<.....<<<<<<<<<......<<<<<<...>>>>>>.
......>>>>>>>.>.>.>>>>>.>>>>>>>>>>........<<<....>
>>.....>>>>>>>..<<<........<<<<....>>>>...........
.....<<<..>>>.....>>>.....<<<<<<....<.<<<....>>>.>
<<.<<.<......<<<.<<<<<<.......................>>>>
...<<<<..<<<..>>>..>>>>.....>>>>>.....>.>>.>>..<<<
<.<<<<<.....>>>>>.>>>>....<<<......>>>...<<<<<<<..
<<<<(((.....<<<<<<.....<<.........<<<.<<<<<<<<<<..
.....<<<<<.<<....<<.........>>....>>.........<<...
...>>...>>>>>...>>>>>>>>>>>>>.........))).>>...>>>
>>>.>>>>..>>>>>>><<<<<<<<<...>>>>>>>>>....>>>>>>..
.<<<<<<..<<.....<<<<<<.....>>>>>>...>>.>>>>>>.....
<<<<<..<<......<<<<<.<<.<<<..<<<<<......>>>>>.....
.<.<..<<.<<<<<..........>>>>>.>>.....<<<<<<<...<<<
.<.........>.>>>.................>>>>>>>...<<<..<<
<<.......>>>>..>>>.<<<....>>>...>.>........<.....>
............>>>...>>>>>>>..............<<<<<...<<.
...>>...>>>>>.................<<<...<<<<<..<.<<<<<
<<......>>>>>>>...>.<<<.........>>>.....>>>>>..>>>
......<<<<<<<....>>>.>>>>.....>>.>>>>>....<<<<<<<<
..<<<<.<.<<<<<<...............>>>>>>.><<<<<...<<<<
...<<<<<<<<<<<....>>>>>.>>>>>>..>>>>..>>>>>.<<<<..
.<<<<<...........>>>>>...>>>>.>>>>....>>>>>>>>....
..<<<<<<.<<<.<<....<<..........<<<<<<.....>>>>>>..
....>>.......<<<<<<...<<<<<.<..<<<......>>>..>.>>>
>>.>>>>>>.............>>.>>>.>>>>>>..<<<<<.<......
.......>.....<<<<<<<<<.<<<....<<<<.<..<<<<<.>>>>>.
.>>>>>...>>>....>>>>.>>>>>..>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7oht Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 4.7 Å
• Binding residue (original residue number in PDB): S2 L4
• Binding residue (residue number reindexed from 1): S1 L3

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0042254 ribosome biogenesis
• GO:0110136 protein-RNA complex remodeling

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus

External links

• PDB: RCSB:7oht, PDBe:7oht, PDBj:7oht
• PDBsum: 7oht
• PubMed: 34813592
• UniProt: P25382|NLE1_YEAST Ribosome assembly protein 4 (Gene Name=RSA4)